Prototype Digital Lateral Flow Sensor Using Impact Electrochemistry in a Competitive Binding Assay

This work demonstrates a lateral flow assay concept on the basis of stochastic-impact electrochemistry. To this end, we first elucidate requirements to employ silver nanoparticles as redox-active labels. Then, we present a prototype that utilizes nanoimpacts from biotinylated silver nanoparticles as...

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Veröffentlicht in:ACS sensors 2022-07, Vol.7 (7), p.1967-1976
Hauptverfasser: Weiß, Lennart J. K., Rinklin, Philipp, Thakur, Bhawana, Music, Emir, Url, Heike, Kopic, Inola, Hoven, Darius, Banzet, Marko, von Trotha, Tassilo, Mayer, Dirk, Wolfrum, Bernhard
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Sprache:eng
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Zusammenfassung:This work demonstrates a lateral flow assay concept on the basis of stochastic-impact electrochemistry. To this end, we first elucidate requirements to employ silver nanoparticles as redox-active labels. Then, we present a prototype that utilizes nanoimpacts from biotinylated silver nanoparticles as readouts to detect free biotin in solution based on competitive binding. The detection is performed in a membrane-based microfluidic system, where free biotin and biotinylated particles compete for streptavidin immobilized on embedded latex beads. Excess nanoparticles are then registered downstream at an array of detection electrodes. In this way, we establish a proof of concept that serves as a blueprint for future “digital” lateral flow sensors.
ISSN:2379-3694
2379-3694
DOI:10.1021/acssensors.2c00728