Effects of urbanization intensity on glomalin-related soil protein in Nanchang, China: Influencing factors and implications for greenspace soil improvement

Glomalin-related soil protein (GRSP) is a stable and persistent glycoprotein secreted by arbuscular mycorrhizal fungi that plays an important role in sequestering soil organic carbon (SOC) and improving soil quality. Rapid urbanization disturbs and degrades the soil quality in the greenspace. However, few studies have investigated the effects of urbanization on GRSP and its influencing factors. This study selected impervious surface area as a measure of urbanization intensity. A total of 184 soil samples were collected from the 0–20 cm soil layer in the greenspace of Nanchang, China (505 km2). The GRSP content, soil properties, urban forest characteristics, and land-use configuration were determined. The total GRSP (TG) and easily extractable GRSP (EEG) contents were 2.38 and 0.57 mg g−1, respectively. TG and EEG decreased by 16.22% and 19.35%, respectively, from low to heavy urbanized areas. Moreover, SOC decreased from 39.9 to 1.4 mg g−1, while EEG/SOC and TG/SOC increased by approximately 17% and 34%, respectively, indicating the significant contribution of GRSP to the SOC pool. Pearson and redundancy analysis showed that GRSP was positively correlated with SOC, phosphorus, nitrogen, vegetation richness, and tree height, but negatively correlated with pH, bulk density, and impervious area. The partial least squares path model demonstrated that urbanization affected soil properties, forest characteristics, and land use factors, resulting in GRSP changes. This study clarifies the key factors of urbanization that affect GRSP and provides insight for urban greenspace soil improvement from the new perspective of enhancing the GRSP content. •The first report on the characteristics of GRSP in the red soils in an urban setting.•GRSP, as an inert C pool, plays a crucial role in stabilizing soil C sequestration.•Urbanization indirectly decreased GRSP content via changes in environment factors.•Soil factors and forest characteristics play key roles in the changes of GRSP.•Urban greenspace soil improvement can be considered from the view of improving GRSP.