Profiling the total single-cell transciptome using droplet microfluidics

Profiling the total single-cell transciptome using droplet microfluidics

Current high-throughput single-cell methods detect only a small part of the transcriptome. The workflow presented here integrates molecular analysis and droplet microfluidics to derive total transcriptomic atlases that encompass alternative splicing and non-coding transcripts in large numbers of sin...

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Veröffentlicht in:Nature biotechnology 2022-12, Vol.40 (12), p.1766-1767
Format: Artikel
Sprache:eng
Schlagworte:
631/1647
631/61
Agriculture
Alternative splicing
Bioinformatics
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
Cell cycle
Collaboration
Datasets
Droplets
Gastrulation
Gene expression
Genomics
Life Sciences
Methods
Microfluidic Analytical Techniques
Microfluidics
Organogenesis
Research Briefing
Single-Cell Analysis
Splicing
Transcriptomes
Transcriptomics
Workflow
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Beschreibung
Zusammenfassung:Current high-throughput single-cell methods detect only a small part of the transcriptome. The workflow presented here integrates molecular analysis and droplet microfluidics to derive total transcriptomic atlases that encompass alternative splicing and non-coding transcripts in large numbers of single cells. The utility of this method is demonstrated by analysis of mouse gastrulation and early organogenesis.
ISSN:1087-0156
1546-1696
DOI:10.1038/s41587-022-01370-7