Understanding immunological origins of atopic dermatitis through multi‐omic analysis

Background The pathophysiology of atopic dermatitis (AD) is multifactorial, impacted by individual medical, demographic, environmental, and immunologic factors. This study used multi‐omic analyses to assess how host and microbial factors could contribute to infant AD development. Methods This longitudinal cohort study included 129 term infants, identified as AD (n = 37) or non‐AD (n = 92) using the Infant Feeding Practices‐II survey and review of medical records. Standardized surveys were used to assess medical and demographic traits (gestational age, sex, race, maternal AD, and atopy family history), and environmental exposures (delivery method, maternal tobacco use, pets, breastfeeding duration, and timing of solid food introduction). Saliva was collected at 6 months for multi‐omic assessment of cytokines, microRNAs, mRNAs, and the microbiome. The contribution of each factor to AD status was assessed with logistic regression. Results Medical, demographic, and environmental factors did not differ between AD and non‐AD infants. Five “omic” factors (IL‐8/IL‐6, miR‐375‐3p, miR‐21‐5p, bacterial diversity, and Proteobacteria) differed between groups (p