UHPLC-Q/Orbitrap/MS based chemical fingerprinting and hepatoprotective potential of a medicinal plant, Morinda angustifolia Roxb

Morinda angustifolia Roxb. is used to treat jaundice in the indigenous system of medicine in Bangladesh. The objective of the present study was to assess the hepatoprotective potential and in silico-based identification of the active constituents of M. angustifolia bark extract. In vivo hepatoprotective activity was evaluated in CCl4-induced hepatotoxicity in rats. The secondary metabolite content of the extract was identified by ultra-performance liquid chromatography coupled with a quadrupole orbitrap mass spectrometer (UHPLC-Q/Orbitrap/MS). Furthermore, UHPLC-Q/Orbitrap/MS identified compounds of the extract were assessed for their effect on liver-protective enzymes, namely superoxide dismutase, catalase, and peroxidase, using an in-silico study. The extract revealed significant (p