Description of a novel IncP plasmid harboring blaKPC-2 recovered from a SPM-1-producing Pseudomonas aeruginosa from ST277

The high rates of carbapenem resistance among Brazilian Pseudomonas aeruginosa isolates are mainly associated with the clone ST277 producing the carbapenemase SPM-1. Here, the complete genetic composition of a IncP plasmid harboring blaKPC-2 in isolates of this endemic clone carrying chromosomal blaSPM-1 was described using whole genome sequencing. These results confirm the association of these two carbapenemases in ST277 and also describe the genetic composition of a novel blaKPC-2-plasmid. Considering the fact that this association occurs in a high-risk clone, monitoring the dissemination of this plasmid should be a public health concern. •Three isolates belonging to the high-risk clone ST277 were positive for blaSPM-1 and blaKPC-2.•Two copies of blaKPC-2 were located in an IncP plasmid, similar to plasmids carrying VIM and IMP carbapenemases.•The novel blaKPC-2 context contains only 73 nt from the most commonly associated Tn4401 sequence.