Prior beetroot juice ingestion offsets endothelial dysfunction following prolonged sitting

Nutritional strategies to prevent endothelial dysfunction following prolonged sitting remain largely unknown. Given that beetroot juice (BRJ) ingestion enhances nitric oxide (NO) bioavailability, we aimed to evaluate whether prior BRJ ingestion would prevent sitting-induced endothelial dysfunction in the leg. Eleven healthy young males (n = 7) and females (n = 4) underwent two experimental trials of prolonged sitting with prior: 1) placebo ingestion (PL trial) and 2) BRJ ingestion (BRJ trial). All subjects ingested 140 ml of PL or BRJ (~0.0055 or ~12.8 mmol of nitrate, respectively) immediately before 3 h of sitting. Pre and post-sitting measurement of popliteal artery flow-mediated dilation (FMD) and blood pressure, and blood collection were undertaken. During the sitting period, an hourly assessment of popliteal artery diameter and blood velocity, blood pressure, and blood collection was performed. Popliteal artery blood flow and shear rate were significantly and similarly reduced during the sitting period in both trials (p < 0.001). Plasma nitrate and NOx (total nitrite and nitrate) concentrations were significantly increased relative to baseline in the only BRJ trial, and the overall concentrations were significantly higher in the BRJ trial (p < 0.001). Popliteal artery FMD was significantly reduced after the sitting period in the PL trial (p < 0.05), whereas no reduction was observed in the BRJ trial. Therefore, prior BRJ ingestion would prevent sitting-induced leg endothelial dysfunction via enhancing NO bioavailability.