Biochar-based microbial agent reduces U and Cd accumulation in vegetables and improves rhizosphere microecology

Microbial remediation of heavy metals in soil has been widely studied. However, bioremediation efficiency is limited in practical applications because of nutritional deficiency, low efficiency, and competition with indigenous microorganisms. Herein, we prepared a biochar-based microbial agent (BMA) by immobilizing the microbial agent (MA, containing Bacillus subtilis, Bacillus cereus, and Citrobacter sp.) on biochar for the remediation of U and Cd in soil. The results showed that BMA increased soil organic matter, cation exchange capacity, and fluorescein diacetate hydrolysis activity and dehydrogenase activity by 58.7%, 38.2%, 42.9%, and 51.1%. The availability of U and Cd were significantly decreased by 67.4% and 54.2% in BMA amended soil, thereby reducing their accumulation in vegetables. BMA greatly promoted vegetable growth. Additionally, BMA significantly altered the structure and function of rhizosphere soil microbial communities. Coincidently, more abundant ecologically beneficial bacteria like Nitrospira, Nitrosomonas, Lysobacter, and Bacillus were observed, whereas plant pathogenic fungi like Fusarium and Alternaria reduced in BMA amended soil. The network analysis revealed that BMA amendment increased the tightness and complexity of microbial communities. Importantly, the compatibility of niches and microbial species within co-occurrence network was enhanced after BMA addition. These findings provide a promising strategy for suppressing heavy metal accumulation in vegetables and promoting their growth. [Display omitted] •The synergistic effect of BC and MA enhanced U and Cd immobilization.•The growth of vegetables was promoted by BMA.•The stability of rhizosphere microecology was enhanced by BMA.•Ecologically beneficial bacteria increased in BMA amended soil.•Several plant pathogenic fungi reduced in BMA amended soil.