Media selection for anammox‐based polishing filters: Balancing anammox enrichment and retention with filtration function

Retrofitting conventional denitrification filters into partial denitrification‐anammox (PdNA)‐ or anammox (AnAOB)‐based filters will reduce the needs for external carbon addition. The success of AnAOB‐based filters depends on anammox growth and retention within such filters. Studies have overlooked the importance of media selection and its impact on AnAOB capacity, head loss progression dynamics, and shear conditions applied onto the AnAOB biofilm. The objective of this study was to evaluate viable media types (10 types) that can enhance AnAOB rates for efficient nitrogen removal in filters. Given the higher backwash requirement and lower AnAOB capacity of the conventionally used sand, expanded clay (3–5 mm) was recommended for AnAOB‐based filters in this study. Owing to its surface characteristics, expanded clay had higher AnAOB activity (304‐ vs. 104‐g NH4+‐N/m2/day) and higher AnAOB retention (43% more) than sand. Increasing the iron content of expanded clay to 37% resulted in an increase in zeta potential, which led to 56% more anammox capacity compared to expanded clay with 7% iron content. This work provides insight into the importance of media types in the growth and retention of AnAOB in filters, and this knowledge could be used as basis in the development of PdNA filters. Practitioner Points Expanded clay showed the lowest head loss buildup and most likely will result in longer runtime for full‐scale PdNA applications The highest AnAOB rates were achieved in expanded clay types and sand compared with smaller media typically used in biofiltration Expanded clay resulted in better AnAOB retention under shear, whereas sand could not withstand shear and required more frequent backwashing Expanded clay iron coating enhanced AnAOB enrichment and retention, most likely due to increased surface roughness and/or positive charge This study provided insight into the importance of media types in the growth and retention of anammox in polishing filters. Expanded clay (3–5 mm) was recommended for anammox‐based or partial denitrification‐anammox (PdNA) polishing filters. Owing to its higher surface roughness and higher iron content, expanded clay had three times more anammox activity and 43% more anammox retention than the conventionally used sand.