Influence of chlorhexidine, propolis, pulpal pressure simulation, and aging on dentin bond strength

The present study evaluated the bond strength (μTBS) of dentin treated with chlorhexidine and propolis subjected to simulated pulpal pressure (SPP) and thermocycle aging. One hundred and twenty healthy human molars were sectioned to obtain 2 mm of dentin thickness and were divided into two groups (n = 60): SPP (15 cm H2O) and no SPP (Control group). Dentin surfaces were conditioned with 37% phosphoric acid for 15 s and were divided according to the dentin treatment (n = 20): Control; Chlorhexidine gluconate (0.2% for 30 s) and Propolis (aqueous propolis extract for 30 s). Half of the specimens were submitted to 15,000 thermocycle aging (5 ± 2°C and 55 ± 2°C). The samples were sectioned into beams and submitted to μTBS. Data were analyzed by three‐way ANOVA (SPP × Dentin treatment × Thermocycle aging) and the Tukey's tests (p .05). The SPP and thermocycle aging negatively influenced the bond strength between the dentin and resin; the 0.2% chlorhexidine digluconate and aqueous propolis extract solutions did not interfere in the bond strength between the resin and dentin. The use of chlorhexidine and propolis as a dental treatment may not influence the dentin bond strength, but SPP and thermocycle aging may damage the longitudinal dentin bond strength. Research Highlights Chlorhexidine and propolis as a dental treatment may not influence the dentin bond strength. Simulated pulpal pressure and thermocycle aging may damage the longitudinal dentin bond strength. SEM photomicrograph (×5000) of the dentin surface of the adhesive fracture for the presence of simulated pulp pressure (15 cm H2O) associated with 15,000 thermocycling aging (5 ± 2°C and 55 ± 2°C).