Chemoarchitecture of area prostriata in adult and developing mice: Comparison with presubiculum and parasubiculum

Retrosplenial area 29e, which was a cortical region described mostly in earlier rodent literature, is often included in the dorsal presubiculum (PrSd) or postsubiculum (PoS) in modern literature and commonly used brain atlases. Recent anatomical and molecular studies have revealed that retrosplenial area 29e belongs to the superficial layers of area prostriata, which in primates is found to be important in fast analysis of quickly moving objects in far peripheral visual field. As in primates, the prostriata in rodents adjoins area 29 (granular retrosplenial area), area 30 (agranular retrosplenial area), medial visual cortex, PrSd/PoS, parasubiculum (PaS), and postrhinal cortex (PoR). The present study aims to reveal the chemoarchitecture of the prostriata versus PrSd/PoS or PaS by means of a systematic survey of gene expression patterns in adult and developing mouse brains. First, we find many genes that display differential expression across the prostriata, PrSd/PoS, and PaS and that show obvious laminar expression patterns. Second, we reveal subsets of genes that selectively express in the dorsal or ventral parts of the prostriata, suggesting the existence of at least two subdivisions. Third, we detect some genes that shows differential expression in the prostriata of postnatal mouse brains from adjoining regions, thus enabling identification of the developing area prostriata. Fourth, gene expression difference of the prostriata from the medial primary visual cortex and PoR is also observed. Finally, molecular and connectional features of the prostriata in rodents and nonhuman primates are discussed and compared. The present study investigated the genoarchitecture of the mouse equivalent of area prostriata (Pro), which receives direct inputs from dorsal lateral geniculate nucleus and primary visual cortex (V1). In both adult and postnatal mice, many genes are expressed in layers 2–3 (e.g., Fstl1) or layer 5 (e.g., Lypd1) or both (not shown) of the prostriata, while some other genes are differentially expressed in the dorsal (e.g., Col5a1) and ventral (e.g., Cdh24) subdivisions of the prostriata. These gene expression patterns make the prostriata distinguishable from adjoining regions such as the postsubiculum and V1.