Determination of intracellular ascorbic acid using tandem mass spectrometry

Determination of intracellular ascorbic acid using tandem mass spectrometry

Ascorbic acid is involved in a variety of biological events. Nevertheless, its detailed intracellular behavior remains unexplored due to a lack of sensitive analytical methods. Here we report a method using HPLC-ESI-MS/MS achieving the lowest detection limit ever reported (1 pg), to provide mechanis...

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Veröffentlicht in:Analyst (London) 2022-06, Vol.147 (12), p.264-2643
Hauptverfasser: Abe, Chizumi, Higuchi, Ohki, Matsumoto, Akira, Miyazawa, Taiki
Format: Artikel
Sprache:eng
Schlagworte:
Ascorbic Acid
Chromatography, High Pressure Liquid - methods
Mass spectrometry
Spectrometry, Mass, Electrospray Ionization - methods
Tandem Mass Spectrometry - methods
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Zusammenfassung:Ascorbic acid is involved in a variety of biological events. Nevertheless, its detailed intracellular behavior remains unexplored due to a lack of sensitive analytical methods. Here we report a method using HPLC-ESI-MS/MS achieving the lowest detection limit ever reported (1 pg), to provide mechanistic insight into the intracellular ascorbic acid physiology. Ascorbic acid is involved in a variety of biological events.
ISSN:0003-2654
1364-5528
DOI:10.1039/d1an02160e