Comparison of different particles and methods for magnetic isolation of circulating tumor cells

A more effective method for tumor cell separation from peripheral blood was established. The results of optimized magnetic particles verified by analyzing yield, purity and viability of isolated epithelial tumor cells were compared with a commercial kit for immunomagnetic cell separation. Porous sil...

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Veröffentlicht in:	Journal of magnetism and magnetic materials 2001, Vol.225 (1), p.175-179
Hauptverfasser:	Sieben, S, Bergemann, C, Lübbe, A, Brockmann, B, Rescheleit, D
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Circulating tumor cells Diverse techniques Epithelial tumor cells Fundamental and applied biological sciences. Psychology Immunomagnetic separation Magnetic beads Microspheres Molecular and cellular biology
Online-Zugang:	Volltext
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Zusammenfassung:	A more effective method for tumor cell separation from peripheral blood was established. The results of optimized magnetic particles verified by analyzing yield, purity and viability of isolated epithelial tumor cells were compared with a commercial kit for immunomagnetic cell separation. Porous silica particles of 230 nm were found to give best recovery rates and high viability of extracted cells.
ISSN:	0304-8853
DOI:	10.1016/S0304-8853(00)01248-8