A loofah-inspired scaffold with enhanced mimicking mechanics and tumor cells distribution for in vitro tumor cell culture platform

Tumor cells cultured in a physiologically related three-dimensional (3D) matrix can replicate many basic characteristics of tumor tissue. Tumor tissues are harder than normal, so when using hydrogels for 3D tumor cell culture, attempts have been made to prepare hydrogel scaffolds that mimic the hard...

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Veröffentlicht in:Biomaterials advances 2022-04, Vol.135, p.112672-112672, Article 112672
Hauptverfasser: Liu, Xingxing, Fu, Sijia, Jiao, Yongjie, Hu, Mengbo, Li, Chaojing, Wang, Fujun, Wang, Lu
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Sprache:eng
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Zusammenfassung:Tumor cells cultured in a physiologically related three-dimensional (3D) matrix can replicate many basic characteristics of tumor tissue. Tumor tissues are harder than normal, so when using hydrogels for 3D tumor cell culture, attempts have been made to prepare hydrogel scaffolds that mimic the hardness of tumor tissues without reducing the porosity. In this study, a new 3D loofah-inspired scaffold was developed for prostate cancer cell culture. Since the loofah sponge structure of the spacer fabric, the composite scaffolds had a compression modulus similar to that of natural prostate tumor tissue at a lower hydrogel concentration (0.25% W/V), and also, endowed the scaffold with high porosity (85 ± 2.52%) for mass transfer. The results of in vitro cell experiments showed that the composite scaffold can support tumor cells to form clusters in a short time (3 days). Preliminary chemosensitivity analysis showed that the drug resistance of the composite scaffold was significantly higher than that of two-dimensional (2D) culture and COL scaffold. Therefore, the 3D tumor cell culture scaffold with bionic structures has the potential to be used as a tumor drug screening model. [Display omitted] •A loofah sponge-inspired scaffold was prepared by 3D fabric combined collagen hydrogel.•The scaffold exhibited enhanced mimicking tumor mechanics without reducing the porosity.•The scaffold showed superior proliferation and distribution of prostate tumor cells in its 3D structure.•Multicellular tumor spheroids (MCTs) generated showed dose-dependent responses to paclitaxel and enzalutamide.
ISSN:0928-4931
2772-9508
1873-0191
2772-9508
DOI:10.1016/j.msec.2022.112672