Molecular detection of Brucella spp. in clinical samples of seropositive ruminants in Bosnia and Herzegovina

Molecular detection of Brucella spp. in clinical samples of seropositive ruminants in Bosnia and Herzegovina

Brucellosis is one of the most common zoonotic diseases worldwide that is endemic to Bosnia and Herzegovina (B&H) and remains an emerging public and animal health concern in this country. Diagnostic testing of brucellosis in ruminants in B&H relies exclusively on serological methods. The pre...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Comparative immunology, microbiology and infectious diseases microbiology and infectious diseases, 2022-07, Vol.86, p.101821-101821, Article 101821
Hauptverfasser: Maksimović, Zinka, Jamaković, Amela, Semren, Orjana, Rifatbegović, Maid
Format: Artikel
Sprache:eng
Schlagworte:
Animal health
Animals
Asymptomatic
Brucella
Brucellosis
Cattle
Deoxyribonucleic acid
Diagnostic systems
DNA
Goats
Polymerase chain reaction
Real time
Real-time PCR
Serology
Sheep
Zoonoses
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Brucellosis is one of the most common zoonotic diseases worldwide that is endemic to Bosnia and Herzegovina (B&H) and remains an emerging public and animal health concern in this country. Diagnostic testing of brucellosis in ruminants in B&H relies exclusively on serological methods. The present study was conducted to determine the presence of Brucella spp. in clinical samples of seropositive ruminants by Real-time PCR assay. Of the 135 samples tested, 37% were positive by Real-time PCR. The frequency of detection of Brucella-DNA in the samples collected from aborted animals (n = 20/20; 100%) was significantly higher (P 
ISSN:0147-9571
1878-1667
DOI:10.1016/j.cimid.2022.101821