Applications of capillary electromigration methods for separation and analysis of proteins (2017–mid 2021) – A review

This paper provides a detailed overview of relevant developments in separation and analysis of proteins by capillary electromigration (CE) methods in the period 2017–mid 2021. The presented topics embrace sample preparation, suppression of protein sorption, control of electroosmotic flow, separations of proteins by the particular CE methods and protein identification and quantification by various detection techniques. To illustrate the wide usability of CE methods for protein analysis, the paper shows several novel applications of CE methods, such as quality control and characterization of protein biopharmaceuticals, determination of proteins in complex biomatrices, investigation of posttranslational modification, peptide mapping, interactions of proteins with other (bio)molecules and determination of their physicochemical and biochemical characteristics. [Display omitted] •Developments in separation and analysis of proteins by CE methods are described.•Methodology aspects of CE separations and analyses of proteins are discussed.•Particular CE methods applied for analysis of proteins are reported.•Detection techniques for identification & quantification of proteins are presented.•Wide applicability of CE methods for protein analysis & characterization is shown.