Hydrogenophilus thiooxidans sp. nov., a moderately thermophilic chemotrophic bacterium unable to grow on hydrogen gas, isolated from hot spring microbial mats
A novel thermophilic chemotrophic bacterium, strain SS56 , was isolated from Nakabusa Hot Spring, Japan. The isolate was a rod-shaped (1.5-2.1×0.6-0.8 µm), Gram-stain-negative bacterium. The cells of this strain grew chemoheterotrophically under aerobic and anaerobic conditions. Autotrophic growth w...
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| Veröffentlicht in: | International journal of systematic and evolutionary microbiology 2022-05, Vol.72 (5) |
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| creator | Kawai, Shigeru Ishikawa, Mikako Hanada, Satoshi Haruta, Shin |
| description | A novel thermophilic chemotrophic bacterium, strain SS56
, was isolated from Nakabusa Hot Spring, Japan. The isolate was a rod-shaped (1.5-2.1×0.6-0.8 µm), Gram-stain-negative bacterium. The cells of this strain grew chemoheterotrophically under aerobic and anaerobic conditions. Autotrophic growth was observed with thiosulphate and elemental sulphur under aerobic conditions but not with H
as the electron donor. Heterotrophic growth in the presence of O
occurred on yeast extract, tryptone, polypeptone and organic acids. Strain SS56
used nitrite as an alternative electron acceptor under anaerobic chemoheterotrophic conditions. The isolate grew between 35 and 65 °C, with the optimum at 55 °C. The pH range for growth was pH 6.0-9.0; optimal growth occurred at pH 7.0-8.0. The 16S rRNA gene sequence of strain SS56
was 98.9% identical to that of
TH-1
. The draft genome sequence of 2401804 bp for strain SS56
gave values of 53.7% for digital DNA-DNA hybridization, 92.9% for average nucleotide identity and 93.6% for average amino acid identity compared with the genome sequence of 2223143 bp for
TH-1
. Based on the information described above, strain SS56
(=DSM 111892
=JCM 34254
) is proposed as the type strain of a novel species,
sp. nov. |
| doi_str_mv | 10.1099/ijsem.0.005355 |
| format | Article |
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, was isolated from Nakabusa Hot Spring, Japan. The isolate was a rod-shaped (1.5-2.1×0.6-0.8 µm), Gram-stain-negative bacterium. The cells of this strain grew chemoheterotrophically under aerobic and anaerobic conditions. Autotrophic growth was observed with thiosulphate and elemental sulphur under aerobic conditions but not with H
as the electron donor. Heterotrophic growth in the presence of O
occurred on yeast extract, tryptone, polypeptone and organic acids. Strain SS56
used nitrite as an alternative electron acceptor under anaerobic chemoheterotrophic conditions. The isolate grew between 35 and 65 °C, with the optimum at 55 °C. The pH range for growth was pH 6.0-9.0; optimal growth occurred at pH 7.0-8.0. The 16S rRNA gene sequence of strain SS56
was 98.9% identical to that of
TH-1
. The draft genome sequence of 2401804 bp for strain SS56
gave values of 53.7% for digital DNA-DNA hybridization, 92.9% for average nucleotide identity and 93.6% for average amino acid identity compared with the genome sequence of 2223143 bp for
TH-1
. Based on the information described above, strain SS56
(=DSM 111892
=JCM 34254
) is proposed as the type strain of a novel species,
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, was isolated from Nakabusa Hot Spring, Japan. The isolate was a rod-shaped (1.5-2.1×0.6-0.8 µm), Gram-stain-negative bacterium. The cells of this strain grew chemoheterotrophically under aerobic and anaerobic conditions. Autotrophic growth was observed with thiosulphate and elemental sulphur under aerobic conditions but not with H
as the electron donor. Heterotrophic growth in the presence of O
occurred on yeast extract, tryptone, polypeptone and organic acids. Strain SS56
used nitrite as an alternative electron acceptor under anaerobic chemoheterotrophic conditions. The isolate grew between 35 and 65 °C, with the optimum at 55 °C. The pH range for growth was pH 6.0-9.0; optimal growth occurred at pH 7.0-8.0. The 16S rRNA gene sequence of strain SS56
was 98.9% identical to that of
TH-1
. The draft genome sequence of 2401804 bp for strain SS56
gave values of 53.7% for digital DNA-DNA hybridization, 92.9% for average nucleotide identity and 93.6% for average amino acid identity compared with the genome sequence of 2223143 bp for
TH-1
. Based on the information described above, strain SS56
(=DSM 111892
=JCM 34254
) is proposed as the type strain of a novel species,
sp. nov.</description><subject>Bacteria - genetics</subject><subject>Bacterial Typing Techniques</subject><subject>Base Composition</subject><subject>DNA, Bacterial - genetics</subject><subject>Fatty Acids - chemistry</subject><subject>Hot Springs - microbiology</subject><subject>Hydrogen - metabolism</subject><subject>Phylogeny</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sequence Analysis, DNA</subject><issn>1466-5026</issn><issn>1466-5034</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kU9v1DAQxS0EoqVw5YjmyKEbxo6d3RyrqlCkSlzgHNnOZOMqjre203a_DJ8Vb3fpaf7oN--N9Bj7zLHi2Lbf3H0iX2GFqGql3rBzLptmpbCWb1970ZyxDyndI5YF4nt2VlApRYvn7O_tvo9hS3PYjW5aEuTRhfDsej0nSLsK5vBYXYIGH3qKOtO0LwhF_8I7C3YkH3I8jBaMtpmiWzwsszYTQQ6wjeEJwgzjyQi2Ol2CS2Eqaj0MMXgYQy5m0c1b8M7GYJyewOucPrJ3g54SfTrVC_bn-83v69vV3a8fP6-v7la2bnheaT6gFdw0ZPv1xm74MChU3FhCzc166OvGtty2Vkk0UuJGrI0gVa-5EXwjVH3Bvh51dzE8LJRy512yNE16prCkTjSNUJK3si5odUTLnylFGrryuNdx33HsDpl0L5l02B0zKQdfTtqL8dS_4v9DqP8BgMeMSw</recordid><startdate>20220531</startdate><enddate>20220531</enddate><creator>Kawai, Shigeru</creator><creator>Ishikawa, Mikako</creator><creator>Hanada, Satoshi</creator><creator>Haruta, Shin</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-3705-2459</orcidid></search><sort><creationdate>20220531</creationdate><title>Hydrogenophilus thiooxidans sp. nov., a moderately thermophilic chemotrophic bacterium unable to grow on hydrogen gas, isolated from hot spring microbial mats</title><author>Kawai, Shigeru ; Ishikawa, Mikako ; Hanada, Satoshi ; Haruta, Shin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c361t-a1f0c21b6ecd78c81ff5051bce0a1b7fd36c91c9c540b440827b2e5371b218253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Bacteria - genetics</topic><topic>Bacterial Typing Techniques</topic><topic>Base Composition</topic><topic>DNA, Bacterial - genetics</topic><topic>Fatty Acids - chemistry</topic><topic>Hot Springs - microbiology</topic><topic>Hydrogen - metabolism</topic><topic>Phylogeny</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Sequence Analysis, DNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kawai, Shigeru</creatorcontrib><creatorcontrib>Ishikawa, Mikako</creatorcontrib><creatorcontrib>Hanada, Satoshi</creatorcontrib><creatorcontrib>Haruta, Shin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of systematic and evolutionary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kawai, Shigeru</au><au>Ishikawa, Mikako</au><au>Hanada, Satoshi</au><au>Haruta, Shin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hydrogenophilus thiooxidans sp. nov., a moderately thermophilic chemotrophic bacterium unable to grow on hydrogen gas, isolated from hot spring microbial mats</atitle><jtitle>International journal of systematic and evolutionary microbiology</jtitle><addtitle>Int J Syst Evol Microbiol</addtitle><date>2022-05-31</date><risdate>2022</risdate><volume>72</volume><issue>5</issue><issn>1466-5026</issn><eissn>1466-5034</eissn><abstract>A novel thermophilic chemotrophic bacterium, strain SS56
, was isolated from Nakabusa Hot Spring, Japan. The isolate was a rod-shaped (1.5-2.1×0.6-0.8 µm), Gram-stain-negative bacterium. The cells of this strain grew chemoheterotrophically under aerobic and anaerobic conditions. Autotrophic growth was observed with thiosulphate and elemental sulphur under aerobic conditions but not with H
as the electron donor. Heterotrophic growth in the presence of O
occurred on yeast extract, tryptone, polypeptone and organic acids. Strain SS56
used nitrite as an alternative electron acceptor under anaerobic chemoheterotrophic conditions. The isolate grew between 35 and 65 °C, with the optimum at 55 °C. The pH range for growth was pH 6.0-9.0; optimal growth occurred at pH 7.0-8.0. The 16S rRNA gene sequence of strain SS56
was 98.9% identical to that of
TH-1
. The draft genome sequence of 2401804 bp for strain SS56
gave values of 53.7% for digital DNA-DNA hybridization, 92.9% for average nucleotide identity and 93.6% for average amino acid identity compared with the genome sequence of 2223143 bp for
TH-1
. Based on the information described above, strain SS56
(=DSM 111892
=JCM 34254
) is proposed as the type strain of a novel species,
sp. nov.</abstract><cop>England</cop><pmid>35544290</pmid><doi>10.1099/ijsem.0.005355</doi><orcidid>https://orcid.org/0000-0002-3705-2459</orcidid></addata></record> |
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| ispartof | International journal of systematic and evolutionary microbiology, 2022-05, Vol.72 (5) |
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| recordid | cdi_proquest_miscellaneous_2662541943 |
| source | MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Bacteria - genetics Bacterial Typing Techniques Base Composition DNA, Bacterial - genetics Fatty Acids - chemistry Hot Springs - microbiology Hydrogen - metabolism Phylogeny RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA |
| title | Hydrogenophilus thiooxidans sp. nov., a moderately thermophilic chemotrophic bacterium unable to grow on hydrogen gas, isolated from hot spring microbial mats |
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