Hydrogenophilus thiooxidans sp. nov., a moderately thermophilic chemotrophic bacterium unable to grow on hydrogen gas, isolated from hot spring microbial mats

A novel thermophilic chemotrophic bacterium, strain SS56 , was isolated from Nakabusa Hot Spring, Japan. The isolate was a rod-shaped (1.5-2.1×0.6-0.8 µm), Gram-stain-negative bacterium. The cells of this strain grew chemoheterotrophically under aerobic and anaerobic conditions. Autotrophic growth was observed with thiosulphate and elemental sulphur under aerobic conditions but not with H as the electron donor. Heterotrophic growth in the presence of O occurred on yeast extract, tryptone, polypeptone and organic acids. Strain SS56 used nitrite as an alternative electron acceptor under anaerobic chemoheterotrophic conditions. The isolate grew between 35 and 65 °C, with the optimum at 55 °C. The pH range for growth was pH 6.0-9.0; optimal growth occurred at pH 7.0-8.0. The 16S rRNA gene sequence of strain SS56 was 98.9% identical to that of TH-1 . The draft genome sequence of 2401804 bp for strain SS56 gave values of 53.7% for digital DNA-DNA hybridization, 92.9% for average nucleotide identity and 93.6% for average amino acid identity compared with the genome sequence of 2223143 bp for TH-1 . Based on the information described above, strain SS56 (=DSM 111892 =JCM 34254 ) is proposed as the type strain of a novel species, sp. nov.