The effects of additive solutions on the development of storage lesions in canine platelet concentrates stored at 4°C

Objective To assess platelet storage lesion development as evaluated by measurement of metabolic markers, platelet activation markers, and aggregometry, and determine the occurrence of bacterial growth in platelets stored in platelet additive solution (PAS) at 4°C for 7 days. Design Prospective, ex vivo experimental controlled study. Setting Research laboratory of a university veterinary teaching hospital. Animals Ten units of canine platelet concentrate collected from blood bank donations. Interventions Concentrates were aliquoted into 4 separate bags containing 100% plasma (control) or 30% plasma and 70% of a PAS (Plasma‐Lyte A, Isoplate, or InterSol). Samples were stored at 4°C without agitation. At days 0, 3, 5, and 7, samples were analyzed for platelet count, mean platelet volume, glucose, lactate, lactate dehydrogenase, Po2, Pco2, degree of swirling, aggregate formation, aggregation via light aggregometry, surface P‐selectin via flow cytometry, and bacterial contamination via culture. Measurements and Main Results Development of storage lesions was minimal, demonstrated by maintenance of a mean pH > 7.2 and mean lactate values