Xenobiotic pollution affects transcription of antibiotic resistance and virulence factors in aquatic microcosms

Antibiotic resistance genes (ARGs) and virulence factors (VFs) are critical threats to human health. Their abundance in aquatic ecosystems is maintained and enhanced via selection driven by environmental xenobiotics. However, their activity and expression in these environments under xenobiotic stress remains unknown. Here ARG and VF expression profiles were examined in aquatic microcosms under ciprofloxacin, glyphosate and sertraline hydrochloride treatment. Ciprofloxacin increased total expression of ARGs, particularly multidrug resistance genes. Total expression of ARGs and VFs decreased significantly under glyphosate and sertraline treatments. However, in opportunistic human pathogens, these agents increased expression of both ARGs and VFs. Xenobiotic pollutants, such as the compounds we tested here, have the potential to disrupt microbial ecology, promote resistance, and increase risk to human health. This study systematically evaluated the effects of environmental xenobiotics on transcription of ARGs and VFs, both of which have direct relevance to human health. Transcription of such genes has been overlooked in previous studies. [Display omitted] •ARGs and VFs were selectively expressed under different xenobiotic treatments.•Ciprofloxacin upregulated ARGs that confer resistance to wider range of antibiotics.•Glyphosate and sertraline hydrochloride induced ARGs and VFs expression in pathogens.•ARGs and VFs were co-expressed in the aquatic microbial community.•Changes of ARGs and VFs under xenobiotic treatments disrupted ecological functions.