Sec24C mediates a Golgi‐independent trafficking pathway that is required for tonoplast localisation of ABCC1 and ABCC2
Summary Protein sorting is an essential biological process in all organisms. Trafficking membrane proteins generally relies on the sorting machinery of the Golgi apparatus. However, many proteins have been found to be delivered to target locations via Golgi‐independent pathways, but the mechanisms u...
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Veröffentlicht in: | The New phytologist 2022-08, Vol.235 (4), p.1486-1500 |
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container_title | The New phytologist |
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creator | Lv, Qiao‐Yan Han, Mei‐Ling Gao, Yi‐Qun Zhang, Chu‐Ying Wang, Ya‐Ling Chao, Zhen‐Fei Zhong, Li‐Yuan Chao, Dai‐Yin |
description | Summary
Protein sorting is an essential biological process in all organisms. Trafficking membrane proteins generally relies on the sorting machinery of the Golgi apparatus. However, many proteins have been found to be delivered to target locations via Golgi‐independent pathways, but the mechanisms underlying this delivery system remain unknown.
Here, we report that Sec24C mediates the direct secretory trafficking of the phytochelatin transporters ABCC1 and ABCC2 from the endoplasmic reticulum (ER) to prevacuolar compartments (PVCs) in Arabidopsis thaliana.
Genetic analysis showed that the sec24c mutants are hypersensitive to cadmium (Cd) and arsenic (As) treatments due to mislocalisation of ABCC1 and ABCC2, which results in defects in the vacuole compartmentalisation of the toxic metals. Furthermore, we found that Sec24C recognises ABCC1 and ABCC2 through direct interactions to mediate their exit from the ER to PVCs, which is independent of brefeldin A‐sensitive post‐Golgi trafficking pathway.
These findings expand our understanding of Golgi‐independent trafficking, which also provide key insights regarding the mechanism of tonoplast protein sorting and open a new perspective on the function of Sec24 proteins. |
doi_str_mv | 10.1111/nph.18201 |
format | Article |
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Protein sorting is an essential biological process in all organisms. Trafficking membrane proteins generally relies on the sorting machinery of the Golgi apparatus. However, many proteins have been found to be delivered to target locations via Golgi‐independent pathways, but the mechanisms underlying this delivery system remain unknown.
Here, we report that Sec24C mediates the direct secretory trafficking of the phytochelatin transporters ABCC1 and ABCC2 from the endoplasmic reticulum (ER) to prevacuolar compartments (PVCs) in Arabidopsis thaliana.
Genetic analysis showed that the sec24c mutants are hypersensitive to cadmium (Cd) and arsenic (As) treatments due to mislocalisation of ABCC1 and ABCC2, which results in defects in the vacuole compartmentalisation of the toxic metals. Furthermore, we found that Sec24C recognises ABCC1 and ABCC2 through direct interactions to mediate their exit from the ER to PVCs, which is independent of brefeldin A‐sensitive post‐Golgi trafficking pathway.
These findings expand our understanding of Golgi‐independent trafficking, which also provide key insights regarding the mechanism of tonoplast protein sorting and open a new perspective on the function of Sec24 proteins.</description><identifier>ISSN: 0028-646X</identifier><identifier>EISSN: 1469-8137</identifier><identifier>DOI: 10.1111/nph.18201</identifier><identifier>PMID: 35510797</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Arabidopsis thaliana ; Arsenic ; Biological activity ; Brefeldin A ; Cadmium ; COPII ; Defects ; Endoplasmic reticulum ; Genetic analysis ; Golgi apparatus ; heavy metal ; Heavy metals ; Membrane proteins ; Membrane trafficking ; Metals ; phytochelatin transporters ; protein sorting ; Protein transport ; Proteins ; Trafficking ; vacuoles</subject><ispartof>The New phytologist, 2022-08, Vol.235 (4), p.1486-1500</ispartof><rights>2022 The Authors. © 2022 New Phytologist Foundation</rights><rights>2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.</rights><rights>Copyright © 2022 New Phytologist Trust</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2831-471ba7c95dae507f6d00f19bf5db037bee4ac02387840c7962b4220c1b407bc03</citedby><cites>FETCH-LOGICAL-c2831-471ba7c95dae507f6d00f19bf5db037bee4ac02387840c7962b4220c1b407bc03</cites><orcidid>0000-0003-3440-2772 ; 0000-0002-7773-7778 ; 0000-0002-5673-1908 ; 0000-0003-2832-4800 ; 0000-0003-3526-8384 ; 0000-0002-8590-1806 ; 0000-0003-2497-2915 ; 0000-0002-6492-1824</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fnph.18201$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fnph.18201$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35510797$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lv, Qiao‐Yan</creatorcontrib><creatorcontrib>Han, Mei‐Ling</creatorcontrib><creatorcontrib>Gao, Yi‐Qun</creatorcontrib><creatorcontrib>Zhang, Chu‐Ying</creatorcontrib><creatorcontrib>Wang, Ya‐Ling</creatorcontrib><creatorcontrib>Chao, Zhen‐Fei</creatorcontrib><creatorcontrib>Zhong, Li‐Yuan</creatorcontrib><creatorcontrib>Chao, Dai‐Yin</creatorcontrib><title>Sec24C mediates a Golgi‐independent trafficking pathway that is required for tonoplast localisation of ABCC1 and ABCC2</title><title>The New phytologist</title><addtitle>New Phytol</addtitle><description>Summary
Protein sorting is an essential biological process in all organisms. Trafficking membrane proteins generally relies on the sorting machinery of the Golgi apparatus. However, many proteins have been found to be delivered to target locations via Golgi‐independent pathways, but the mechanisms underlying this delivery system remain unknown.
Here, we report that Sec24C mediates the direct secretory trafficking of the phytochelatin transporters ABCC1 and ABCC2 from the endoplasmic reticulum (ER) to prevacuolar compartments (PVCs) in Arabidopsis thaliana.
Genetic analysis showed that the sec24c mutants are hypersensitive to cadmium (Cd) and arsenic (As) treatments due to mislocalisation of ABCC1 and ABCC2, which results in defects in the vacuole compartmentalisation of the toxic metals. Furthermore, we found that Sec24C recognises ABCC1 and ABCC2 through direct interactions to mediate their exit from the ER to PVCs, which is independent of brefeldin A‐sensitive post‐Golgi trafficking pathway.
These findings expand our understanding of Golgi‐independent trafficking, which also provide key insights regarding the mechanism of tonoplast protein sorting and open a new perspective on the function of Sec24 proteins.</description><subject>Arabidopsis thaliana</subject><subject>Arsenic</subject><subject>Biological activity</subject><subject>Brefeldin A</subject><subject>Cadmium</subject><subject>COPII</subject><subject>Defects</subject><subject>Endoplasmic reticulum</subject><subject>Genetic analysis</subject><subject>Golgi apparatus</subject><subject>heavy metal</subject><subject>Heavy metals</subject><subject>Membrane proteins</subject><subject>Membrane trafficking</subject><subject>Metals</subject><subject>phytochelatin transporters</subject><subject>protein sorting</subject><subject>Protein transport</subject><subject>Proteins</subject><subject>Trafficking</subject><subject>vacuoles</subject><issn>0028-646X</issn><issn>1469-8137</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp10UFO3DAUBmCrApUpdNELVJa6gUXg2XHiZAkRMEgjWgmQuoscx2ZMM3awHcHseoSesSfBMLQLJN7C9uLTL-v9CH0hcEjSHNlxeUgqCuQDmhFW1llFcr6FZgC0ykpW_txBn0K4A4C6KOlHtJMXBQFe8xl6vFKSsgavVG9EVAELfO6GW_P39x9jezWqdNiIoxdaG_nL2Fs8irh8EGsclyJiE7BX95PxqsfaeRyddeMgQsSDk2IwQUTjLHYaH580DcHC9i8vuoe2tRiC-vx676Kbs9PrZp4tvp9fNMeLTNIqJxnjpBNc1kUvVAFclz2AJnWni76DnHdKMSGB5hWvGEhel7RjlIIkHQPeSch30f4md_TuflIhtisTpBoGYZWbQkvLEghQgDzRb2_onZu8Tb9LqkrDWE2SOtgo6V0IXul29GYl_Lol0D7X0aY62pc6kv36mjh1acP_5b_9J3C0AQ9mUOv3k9rLH_NN5BPiOZOt</recordid><startdate>202208</startdate><enddate>202208</enddate><creator>Lv, Qiao‐Yan</creator><creator>Han, Mei‐Ling</creator><creator>Gao, Yi‐Qun</creator><creator>Zhang, Chu‐Ying</creator><creator>Wang, Ya‐Ling</creator><creator>Chao, Zhen‐Fei</creator><creator>Zhong, Li‐Yuan</creator><creator>Chao, Dai‐Yin</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7SN</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-3440-2772</orcidid><orcidid>https://orcid.org/0000-0002-7773-7778</orcidid><orcidid>https://orcid.org/0000-0002-5673-1908</orcidid><orcidid>https://orcid.org/0000-0003-2832-4800</orcidid><orcidid>https://orcid.org/0000-0003-3526-8384</orcidid><orcidid>https://orcid.org/0000-0002-8590-1806</orcidid><orcidid>https://orcid.org/0000-0003-2497-2915</orcidid><orcidid>https://orcid.org/0000-0002-6492-1824</orcidid></search><sort><creationdate>202208</creationdate><title>Sec24C mediates a Golgi‐independent trafficking pathway that is required for tonoplast localisation of ABCC1 and ABCC2</title><author>Lv, Qiao‐Yan ; Han, Mei‐Ling ; Gao, Yi‐Qun ; Zhang, Chu‐Ying ; Wang, Ya‐Ling ; Chao, Zhen‐Fei ; Zhong, Li‐Yuan ; Chao, Dai‐Yin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2831-471ba7c95dae507f6d00f19bf5db037bee4ac02387840c7962b4220c1b407bc03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Arabidopsis thaliana</topic><topic>Arsenic</topic><topic>Biological activity</topic><topic>Brefeldin A</topic><topic>Cadmium</topic><topic>COPII</topic><topic>Defects</topic><topic>Endoplasmic reticulum</topic><topic>Genetic analysis</topic><topic>Golgi apparatus</topic><topic>heavy metal</topic><topic>Heavy metals</topic><topic>Membrane proteins</topic><topic>Membrane trafficking</topic><topic>Metals</topic><topic>phytochelatin transporters</topic><topic>protein sorting</topic><topic>Protein transport</topic><topic>Proteins</topic><topic>Trafficking</topic><topic>vacuoles</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lv, Qiao‐Yan</creatorcontrib><creatorcontrib>Han, Mei‐Ling</creatorcontrib><creatorcontrib>Gao, Yi‐Qun</creatorcontrib><creatorcontrib>Zhang, Chu‐Ying</creatorcontrib><creatorcontrib>Wang, Ya‐Ling</creatorcontrib><creatorcontrib>Chao, Zhen‐Fei</creatorcontrib><creatorcontrib>Zhong, Li‐Yuan</creatorcontrib><creatorcontrib>Chao, Dai‐Yin</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The New phytologist</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lv, Qiao‐Yan</au><au>Han, Mei‐Ling</au><au>Gao, Yi‐Qun</au><au>Zhang, Chu‐Ying</au><au>Wang, Ya‐Ling</au><au>Chao, Zhen‐Fei</au><au>Zhong, Li‐Yuan</au><au>Chao, Dai‐Yin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sec24C mediates a Golgi‐independent trafficking pathway that is required for tonoplast localisation of ABCC1 and ABCC2</atitle><jtitle>The New phytologist</jtitle><addtitle>New Phytol</addtitle><date>2022-08</date><risdate>2022</risdate><volume>235</volume><issue>4</issue><spage>1486</spage><epage>1500</epage><pages>1486-1500</pages><issn>0028-646X</issn><eissn>1469-8137</eissn><abstract>Summary
Protein sorting is an essential biological process in all organisms. Trafficking membrane proteins generally relies on the sorting machinery of the Golgi apparatus. However, many proteins have been found to be delivered to target locations via Golgi‐independent pathways, but the mechanisms underlying this delivery system remain unknown.
Here, we report that Sec24C mediates the direct secretory trafficking of the phytochelatin transporters ABCC1 and ABCC2 from the endoplasmic reticulum (ER) to prevacuolar compartments (PVCs) in Arabidopsis thaliana.
Genetic analysis showed that the sec24c mutants are hypersensitive to cadmium (Cd) and arsenic (As) treatments due to mislocalisation of ABCC1 and ABCC2, which results in defects in the vacuole compartmentalisation of the toxic metals. Furthermore, we found that Sec24C recognises ABCC1 and ABCC2 through direct interactions to mediate their exit from the ER to PVCs, which is independent of brefeldin A‐sensitive post‐Golgi trafficking pathway.
These findings expand our understanding of Golgi‐independent trafficking, which also provide key insights regarding the mechanism of tonoplast protein sorting and open a new perspective on the function of Sec24 proteins.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>35510797</pmid><doi>10.1111/nph.18201</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0003-3440-2772</orcidid><orcidid>https://orcid.org/0000-0002-7773-7778</orcidid><orcidid>https://orcid.org/0000-0002-5673-1908</orcidid><orcidid>https://orcid.org/0000-0003-2832-4800</orcidid><orcidid>https://orcid.org/0000-0003-3526-8384</orcidid><orcidid>https://orcid.org/0000-0002-8590-1806</orcidid><orcidid>https://orcid.org/0000-0003-2497-2915</orcidid><orcidid>https://orcid.org/0000-0002-6492-1824</orcidid></addata></record> |
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source | Wiley Free Content; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
subjects | Arabidopsis thaliana Arsenic Biological activity Brefeldin A Cadmium COPII Defects Endoplasmic reticulum Genetic analysis Golgi apparatus heavy metal Heavy metals Membrane proteins Membrane trafficking Metals phytochelatin transporters protein sorting Protein transport Proteins Trafficking vacuoles |
title | Sec24C mediates a Golgi‐independent trafficking pathway that is required for tonoplast localisation of ABCC1 and ABCC2 |
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