Sec24C mediates a Golgi‐independent trafficking pathway that is required for tonoplast localisation of ABCC1 and ABCC2

Summary Protein sorting is an essential biological process in all organisms. Trafficking membrane proteins generally relies on the sorting machinery of the Golgi apparatus. However, many proteins have been found to be delivered to target locations via Golgi‐independent pathways, but the mechanisms u...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:The New phytologist 2022-08, Vol.235 (4), p.1486-1500
Hauptverfasser: Lv, Qiao‐Yan, Han, Mei‐Ling, Gao, Yi‐Qun, Zhang, Chu‐Ying, Wang, Ya‐Ling, Chao, Zhen‐Fei, Zhong, Li‐Yuan, Chao, Dai‐Yin
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 1500
container_issue 4
container_start_page 1486
container_title The New phytologist
container_volume 235
creator Lv, Qiao‐Yan
Han, Mei‐Ling
Gao, Yi‐Qun
Zhang, Chu‐Ying
Wang, Ya‐Ling
Chao, Zhen‐Fei
Zhong, Li‐Yuan
Chao, Dai‐Yin
description Summary Protein sorting is an essential biological process in all organisms. Trafficking membrane proteins generally relies on the sorting machinery of the Golgi apparatus. However, many proteins have been found to be delivered to target locations via Golgi‐independent pathways, but the mechanisms underlying this delivery system remain unknown. Here, we report that Sec24C mediates the direct secretory trafficking of the phytochelatin transporters ABCC1 and ABCC2 from the endoplasmic reticulum (ER) to prevacuolar compartments (PVCs) in Arabidopsis thaliana. Genetic analysis showed that the sec24c mutants are hypersensitive to cadmium (Cd) and arsenic (As) treatments due to mislocalisation of ABCC1 and ABCC2, which results in defects in the vacuole compartmentalisation of the toxic metals. Furthermore, we found that Sec24C recognises ABCC1 and ABCC2 through direct interactions to mediate their exit from the ER to PVCs, which is independent of brefeldin A‐sensitive post‐Golgi trafficking pathway. These findings expand our understanding of Golgi‐independent trafficking, which also provide key insights regarding the mechanism of tonoplast protein sorting and open a new perspective on the function of Sec24 proteins.
doi_str_mv 10.1111/nph.18201
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2660102003</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2660102003</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2831-471ba7c95dae507f6d00f19bf5db037bee4ac02387840c7962b4220c1b407bc03</originalsourceid><addsrcrecordid>eNp10UFO3DAUBmCrApUpdNELVJa6gUXg2XHiZAkRMEgjWgmQuoscx2ZMM3awHcHseoSesSfBMLQLJN7C9uLTL-v9CH0hcEjSHNlxeUgqCuQDmhFW1llFcr6FZgC0ykpW_txBn0K4A4C6KOlHtJMXBQFe8xl6vFKSsgavVG9EVAELfO6GW_P39x9jezWqdNiIoxdaG_nL2Fs8irh8EGsclyJiE7BX95PxqsfaeRyddeMgQsSDk2IwQUTjLHYaH580DcHC9i8vuoe2tRiC-vx676Kbs9PrZp4tvp9fNMeLTNIqJxnjpBNc1kUvVAFclz2AJnWni76DnHdKMSGB5hWvGEhel7RjlIIkHQPeSch30f4md_TuflIhtisTpBoGYZWbQkvLEghQgDzRb2_onZu8Tb9LqkrDWE2SOtgo6V0IXul29GYl_Lol0D7X0aY62pc6kv36mjh1acP_5b_9J3C0AQ9mUOv3k9rLH_NN5BPiOZOt</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2688884491</pqid></control><display><type>article</type><title>Sec24C mediates a Golgi‐independent trafficking pathway that is required for tonoplast localisation of ABCC1 and ABCC2</title><source>Wiley Free Content</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Lv, Qiao‐Yan ; Han, Mei‐Ling ; Gao, Yi‐Qun ; Zhang, Chu‐Ying ; Wang, Ya‐Ling ; Chao, Zhen‐Fei ; Zhong, Li‐Yuan ; Chao, Dai‐Yin</creator><creatorcontrib>Lv, Qiao‐Yan ; Han, Mei‐Ling ; Gao, Yi‐Qun ; Zhang, Chu‐Ying ; Wang, Ya‐Ling ; Chao, Zhen‐Fei ; Zhong, Li‐Yuan ; Chao, Dai‐Yin</creatorcontrib><description>Summary Protein sorting is an essential biological process in all organisms. Trafficking membrane proteins generally relies on the sorting machinery of the Golgi apparatus. However, many proteins have been found to be delivered to target locations via Golgi‐independent pathways, but the mechanisms underlying this delivery system remain unknown. Here, we report that Sec24C mediates the direct secretory trafficking of the phytochelatin transporters ABCC1 and ABCC2 from the endoplasmic reticulum (ER) to prevacuolar compartments (PVCs) in Arabidopsis thaliana. Genetic analysis showed that the sec24c mutants are hypersensitive to cadmium (Cd) and arsenic (As) treatments due to mislocalisation of ABCC1 and ABCC2, which results in defects in the vacuole compartmentalisation of the toxic metals. Furthermore, we found that Sec24C recognises ABCC1 and ABCC2 through direct interactions to mediate their exit from the ER to PVCs, which is independent of brefeldin A‐sensitive post‐Golgi trafficking pathway. These findings expand our understanding of Golgi‐independent trafficking, which also provide key insights regarding the mechanism of tonoplast protein sorting and open a new perspective on the function of Sec24 proteins.</description><identifier>ISSN: 0028-646X</identifier><identifier>EISSN: 1469-8137</identifier><identifier>DOI: 10.1111/nph.18201</identifier><identifier>PMID: 35510797</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Arabidopsis thaliana ; Arsenic ; Biological activity ; Brefeldin A ; Cadmium ; COPII ; Defects ; Endoplasmic reticulum ; Genetic analysis ; Golgi apparatus ; heavy metal ; Heavy metals ; Membrane proteins ; Membrane trafficking ; Metals ; phytochelatin transporters ; protein sorting ; Protein transport ; Proteins ; Trafficking ; vacuoles</subject><ispartof>The New phytologist, 2022-08, Vol.235 (4), p.1486-1500</ispartof><rights>2022 The Authors. © 2022 New Phytologist Foundation</rights><rights>2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.</rights><rights>Copyright © 2022 New Phytologist Trust</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2831-471ba7c95dae507f6d00f19bf5db037bee4ac02387840c7962b4220c1b407bc03</citedby><cites>FETCH-LOGICAL-c2831-471ba7c95dae507f6d00f19bf5db037bee4ac02387840c7962b4220c1b407bc03</cites><orcidid>0000-0003-3440-2772 ; 0000-0002-7773-7778 ; 0000-0002-5673-1908 ; 0000-0003-2832-4800 ; 0000-0003-3526-8384 ; 0000-0002-8590-1806 ; 0000-0003-2497-2915 ; 0000-0002-6492-1824</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fnph.18201$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fnph.18201$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35510797$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lv, Qiao‐Yan</creatorcontrib><creatorcontrib>Han, Mei‐Ling</creatorcontrib><creatorcontrib>Gao, Yi‐Qun</creatorcontrib><creatorcontrib>Zhang, Chu‐Ying</creatorcontrib><creatorcontrib>Wang, Ya‐Ling</creatorcontrib><creatorcontrib>Chao, Zhen‐Fei</creatorcontrib><creatorcontrib>Zhong, Li‐Yuan</creatorcontrib><creatorcontrib>Chao, Dai‐Yin</creatorcontrib><title>Sec24C mediates a Golgi‐independent trafficking pathway that is required for tonoplast localisation of ABCC1 and ABCC2</title><title>The New phytologist</title><addtitle>New Phytol</addtitle><description>Summary Protein sorting is an essential biological process in all organisms. Trafficking membrane proteins generally relies on the sorting machinery of the Golgi apparatus. However, many proteins have been found to be delivered to target locations via Golgi‐independent pathways, but the mechanisms underlying this delivery system remain unknown. Here, we report that Sec24C mediates the direct secretory trafficking of the phytochelatin transporters ABCC1 and ABCC2 from the endoplasmic reticulum (ER) to prevacuolar compartments (PVCs) in Arabidopsis thaliana. Genetic analysis showed that the sec24c mutants are hypersensitive to cadmium (Cd) and arsenic (As) treatments due to mislocalisation of ABCC1 and ABCC2, which results in defects in the vacuole compartmentalisation of the toxic metals. Furthermore, we found that Sec24C recognises ABCC1 and ABCC2 through direct interactions to mediate their exit from the ER to PVCs, which is independent of brefeldin A‐sensitive post‐Golgi trafficking pathway. These findings expand our understanding of Golgi‐independent trafficking, which also provide key insights regarding the mechanism of tonoplast protein sorting and open a new perspective on the function of Sec24 proteins.</description><subject>Arabidopsis thaliana</subject><subject>Arsenic</subject><subject>Biological activity</subject><subject>Brefeldin A</subject><subject>Cadmium</subject><subject>COPII</subject><subject>Defects</subject><subject>Endoplasmic reticulum</subject><subject>Genetic analysis</subject><subject>Golgi apparatus</subject><subject>heavy metal</subject><subject>Heavy metals</subject><subject>Membrane proteins</subject><subject>Membrane trafficking</subject><subject>Metals</subject><subject>phytochelatin transporters</subject><subject>protein sorting</subject><subject>Protein transport</subject><subject>Proteins</subject><subject>Trafficking</subject><subject>vacuoles</subject><issn>0028-646X</issn><issn>1469-8137</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp10UFO3DAUBmCrApUpdNELVJa6gUXg2XHiZAkRMEgjWgmQuoscx2ZMM3awHcHseoSesSfBMLQLJN7C9uLTL-v9CH0hcEjSHNlxeUgqCuQDmhFW1llFcr6FZgC0ykpW_txBn0K4A4C6KOlHtJMXBQFe8xl6vFKSsgavVG9EVAELfO6GW_P39x9jezWqdNiIoxdaG_nL2Fs8irh8EGsclyJiE7BX95PxqsfaeRyddeMgQsSDk2IwQUTjLHYaH580DcHC9i8vuoe2tRiC-vx676Kbs9PrZp4tvp9fNMeLTNIqJxnjpBNc1kUvVAFclz2AJnWni76DnHdKMSGB5hWvGEhel7RjlIIkHQPeSch30f4md_TuflIhtisTpBoGYZWbQkvLEghQgDzRb2_onZu8Tb9LqkrDWE2SOtgo6V0IXul29GYl_Lol0D7X0aY62pc6kv36mjh1acP_5b_9J3C0AQ9mUOv3k9rLH_NN5BPiOZOt</recordid><startdate>202208</startdate><enddate>202208</enddate><creator>Lv, Qiao‐Yan</creator><creator>Han, Mei‐Ling</creator><creator>Gao, Yi‐Qun</creator><creator>Zhang, Chu‐Ying</creator><creator>Wang, Ya‐Ling</creator><creator>Chao, Zhen‐Fei</creator><creator>Zhong, Li‐Yuan</creator><creator>Chao, Dai‐Yin</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7SN</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-3440-2772</orcidid><orcidid>https://orcid.org/0000-0002-7773-7778</orcidid><orcidid>https://orcid.org/0000-0002-5673-1908</orcidid><orcidid>https://orcid.org/0000-0003-2832-4800</orcidid><orcidid>https://orcid.org/0000-0003-3526-8384</orcidid><orcidid>https://orcid.org/0000-0002-8590-1806</orcidid><orcidid>https://orcid.org/0000-0003-2497-2915</orcidid><orcidid>https://orcid.org/0000-0002-6492-1824</orcidid></search><sort><creationdate>202208</creationdate><title>Sec24C mediates a Golgi‐independent trafficking pathway that is required for tonoplast localisation of ABCC1 and ABCC2</title><author>Lv, Qiao‐Yan ; Han, Mei‐Ling ; Gao, Yi‐Qun ; Zhang, Chu‐Ying ; Wang, Ya‐Ling ; Chao, Zhen‐Fei ; Zhong, Li‐Yuan ; Chao, Dai‐Yin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2831-471ba7c95dae507f6d00f19bf5db037bee4ac02387840c7962b4220c1b407bc03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Arabidopsis thaliana</topic><topic>Arsenic</topic><topic>Biological activity</topic><topic>Brefeldin A</topic><topic>Cadmium</topic><topic>COPII</topic><topic>Defects</topic><topic>Endoplasmic reticulum</topic><topic>Genetic analysis</topic><topic>Golgi apparatus</topic><topic>heavy metal</topic><topic>Heavy metals</topic><topic>Membrane proteins</topic><topic>Membrane trafficking</topic><topic>Metals</topic><topic>phytochelatin transporters</topic><topic>protein sorting</topic><topic>Protein transport</topic><topic>Proteins</topic><topic>Trafficking</topic><topic>vacuoles</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lv, Qiao‐Yan</creatorcontrib><creatorcontrib>Han, Mei‐Ling</creatorcontrib><creatorcontrib>Gao, Yi‐Qun</creatorcontrib><creatorcontrib>Zhang, Chu‐Ying</creatorcontrib><creatorcontrib>Wang, Ya‐Ling</creatorcontrib><creatorcontrib>Chao, Zhen‐Fei</creatorcontrib><creatorcontrib>Zhong, Li‐Yuan</creatorcontrib><creatorcontrib>Chao, Dai‐Yin</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) 1: Biological Sciences &amp; Living Resources</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The New phytologist</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lv, Qiao‐Yan</au><au>Han, Mei‐Ling</au><au>Gao, Yi‐Qun</au><au>Zhang, Chu‐Ying</au><au>Wang, Ya‐Ling</au><au>Chao, Zhen‐Fei</au><au>Zhong, Li‐Yuan</au><au>Chao, Dai‐Yin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sec24C mediates a Golgi‐independent trafficking pathway that is required for tonoplast localisation of ABCC1 and ABCC2</atitle><jtitle>The New phytologist</jtitle><addtitle>New Phytol</addtitle><date>2022-08</date><risdate>2022</risdate><volume>235</volume><issue>4</issue><spage>1486</spage><epage>1500</epage><pages>1486-1500</pages><issn>0028-646X</issn><eissn>1469-8137</eissn><abstract>Summary Protein sorting is an essential biological process in all organisms. Trafficking membrane proteins generally relies on the sorting machinery of the Golgi apparatus. However, many proteins have been found to be delivered to target locations via Golgi‐independent pathways, but the mechanisms underlying this delivery system remain unknown. Here, we report that Sec24C mediates the direct secretory trafficking of the phytochelatin transporters ABCC1 and ABCC2 from the endoplasmic reticulum (ER) to prevacuolar compartments (PVCs) in Arabidopsis thaliana. Genetic analysis showed that the sec24c mutants are hypersensitive to cadmium (Cd) and arsenic (As) treatments due to mislocalisation of ABCC1 and ABCC2, which results in defects in the vacuole compartmentalisation of the toxic metals. Furthermore, we found that Sec24C recognises ABCC1 and ABCC2 through direct interactions to mediate their exit from the ER to PVCs, which is independent of brefeldin A‐sensitive post‐Golgi trafficking pathway. These findings expand our understanding of Golgi‐independent trafficking, which also provide key insights regarding the mechanism of tonoplast protein sorting and open a new perspective on the function of Sec24 proteins.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>35510797</pmid><doi>10.1111/nph.18201</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0003-3440-2772</orcidid><orcidid>https://orcid.org/0000-0002-7773-7778</orcidid><orcidid>https://orcid.org/0000-0002-5673-1908</orcidid><orcidid>https://orcid.org/0000-0003-2832-4800</orcidid><orcidid>https://orcid.org/0000-0003-3526-8384</orcidid><orcidid>https://orcid.org/0000-0002-8590-1806</orcidid><orcidid>https://orcid.org/0000-0003-2497-2915</orcidid><orcidid>https://orcid.org/0000-0002-6492-1824</orcidid></addata></record>
fulltext fulltext
identifier ISSN: 0028-646X
ispartof The New phytologist, 2022-08, Vol.235 (4), p.1486-1500
issn 0028-646X
1469-8137
language eng
recordid cdi_proquest_miscellaneous_2660102003
source Wiley Free Content; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals
subjects Arabidopsis thaliana
Arsenic
Biological activity
Brefeldin A
Cadmium
COPII
Defects
Endoplasmic reticulum
Genetic analysis
Golgi apparatus
heavy metal
Heavy metals
Membrane proteins
Membrane trafficking
Metals
phytochelatin transporters
protein sorting
Protein transport
Proteins
Trafficking
vacuoles
title Sec24C mediates a Golgi‐independent trafficking pathway that is required for tonoplast localisation of ABCC1 and ABCC2
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-11T09%3A21%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Sec24C%20mediates%20a%20Golgi%E2%80%90independent%20trafficking%20pathway%20that%20is%20required%20for%20tonoplast%20localisation%20of%20ABCC1%20and%20ABCC2&rft.jtitle=The%20New%20phytologist&rft.au=Lv,%20Qiao%E2%80%90Yan&rft.date=2022-08&rft.volume=235&rft.issue=4&rft.spage=1486&rft.epage=1500&rft.pages=1486-1500&rft.issn=0028-646X&rft.eissn=1469-8137&rft_id=info:doi/10.1111/nph.18201&rft_dat=%3Cproquest_cross%3E2660102003%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2688884491&rft_id=info:pmid/35510797&rfr_iscdi=true