Chryseobacterium subflavum sp. nov., isolated from soil

A Gram-stain-negative, aerobic, non-motile, rod-shaped bacterium, designated LAMRS1 , was isolated from a soil sample collected in Hebei Province, PR China. Strain LAMRS1 was able to grow optimally in the presence of 0.5 % (w/v) NaCl, at pH 7.5 and at 30 °C. On the basis of 16S rRNA gene sequence analysis, strain LAMRS1 was closely related to members of the genus , with highest levels of sequence similarity to DSM 19298 (97.9 %), DSM 17072 (97.6%) and CTM (97.5 %). The average nucleotide identity and digital DNA-DNA hybridization values between LAMRS1 and the closely related species of DSM 19298 , DSM 17072 and CTM were 78.1, 78.2 and 80.7 %, and 21.7, 22.0 and 23.7 %, respectively. The draft genome sequence of LAMRS1 was 4.61 Mb, with DNA G+C content of 36.2 mol%. The major isoprenoid quinone was menaquinone-6 and iso-C , iso-C 3-OH and summed feature 3 (C 6 and/or C 7 ) constituted the major cellular fatty acids. The main polar lipids were phosphatidylethanolamine, four aminolipids, three glycolipids and seven unidentified lipids. On the basis of evidence presented in this study, strain LAMRS1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is LAMRS1 (=JCM 33868 =KCTC 72823 ).