Concentrating forensic DNA sample extracts on the Microlab® STARlet using the Microlab® monitored multi-flow, positive pressure, evaporative extraction module unit

A Microlab® Monitored Multi-Flow, Positive Pressure, Evaporative Extraction module ([MPE]2) unit (Hamilton Company, Reno, Nevada, USA) was installed on the Microlab® STARlet Automated Liquid Handler (Hamilton Company, Reno, Nevada, USA) to add sample concentrating capabilities to the Centre of Forensic Sciences’ automated workflow. Prior to incorporation of the [MPE]2, forensic samples extracted on the STARlet that required concentration to meet the CFS’ amplification threshold were not amplified. Filtering parameters were first optimized, then contamination was assessed, and mock casework studies were completed. There was no evidence of cross contamination or sample loss during sample concentration on the [MPE]2. Extracts from blood, envelope flaps, cigarette butts and drink container swabs were concentrated using the [MPE]2 and amplified using AmpFLSTR™ Identifiler™ Plus (Applied Biosystems™). Profiles were concordant with similar peak heights, whether concentrated manually or with the [MPE]2. Post validation, the [MPE]2 was successfully introduced into casework and in the first year an additional 450 DNA profiles, which previously would not have been amplified, were uploaded to Canada’s National DNA Databank. •Automated concentration of forensic gDNA was achieved using the [MPE]2.•STR profiles were comparable between manually versus [MPE]2 concentrated samples.•No contamination was introduced during sample concentration.