Role of TLR9 methylation on its active transcription in apical inflammation

Aim To explore the methylation pattern, its role in transcriptional regulation and potential modifiers of methylation of the TLR9 gene in chronic periapical inflammation. Methodology In this cross‐sectional study, apical lesions of endodontic origin (ALEO, n = 61) and healthy periodontal ligaments (...

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Veröffentlicht in:	International endodontic journal 2022-07, Vol.55 (7), p.784-794
Hauptverfasser:	Fernández, Alejandra, Astorga, Jessica, Bordagaray, María José, Lira, María Jesús, Gebicke‐Haerter, Peter J., Hernández, Marcela
Format:	Artikel
Sprache:	eng
Schlagworte:	Aging CpG island CpG islands CpG Islands - genetics Cross-Sectional Studies Demethylation DNA Methylation Gene regulation Gum disease Humans infection Inflammation Multivariate analysis periapical periodontitis RNA messenger RNA, Messenger - metabolism TLR9 protein Toll-Like Receptor 9 - genetics Toll-Like Receptor 9 - metabolism Toll-like receptors toll‐like receptor 9
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Zusammenfassung:	Aim To explore the methylation pattern, its role in transcriptional regulation and potential modifiers of methylation of the TLR9 gene in chronic periapical inflammation. Methodology In this cross‐sectional study, apical lesions of endodontic origin (ALEO, n = 61) and healthy periodontal ligaments (HPL, n = 15) were included. Products from bisulfited and PCR‐amplified DNA were analysed for their methylation profiles in the promoter region and at each CpG island. Additionally, TLR9 mRNA levels were quantified by qPCR and bivariate and multiple modelling were performed to better understand the influence of methylation on gene transcription. Results TLR9 mRNA levels were upregulated in ALEO compared to HPL (p
ISSN:	0143-2885 1365-2591
DOI:	10.1111/iej.13745