Insights into protein-ionic liquid interaction: A comprehensive overview on theoretical and experimental approaches

Owing to highly tunable nature, ionic liquids are nesting stance in the scientific community for a wide variety of applications ranging from electrochemistry to product purification, from chemical and biomedical applications to biotechnological interventions and proteomics. Proteins are unstable in its native form and several attempts have been made to stabilize them by addition of various additives. This review focusses on the studies conducted to improve protein stability with ionic liquids along with an emphasis on the mechanism of interaction. This review also specifies and discusses about the brief introduction to ionic liquids, evolution of first-, second-, and third generation of liquids over the years and their selection criterion and applications. Though, there are several elegant reviews available on proteins-ionic liquids interaction, this review systematically highlights the effect of ionic liquids viz., imidazolium, ammonium, phosphonium and choline-based ionic liquids (amino acid-based anions & classical anions) on fibrous proteins viz., collagen and keratin and globular proteins viz., bovine serum albumin and cytochrome c. Thus, this review elaborates the thorough investigations conducted to explore the stabilizing properties of ionic liquids over fibrous and globular proteins.