Encapsulation of Bacillus pumilus G5 from polyvinyl alcohol‑sodium alginate (PVA-SA) and its implications in improving plant growth and soil fertility under drought and salt soil conditions

Drought and salt stresses adversely affect the growth and yield of plants in agricultural production. Bacillus pumilus, an important plant growth-promoting bacterium, play a significant role in improving plant tolerance to abiotic stresses. In this study, B. pumilus G5 were immobilized in polyvinyl alcohol‑sodium alginate (PVA-SA) microbeads and then applied on the Pharbitis nil under drought and salt stresses by pot experiment. Orthogonal array experiments showed that the optimal immobilization conditions of PVA-SA immobilized G5 microbeads were adsorbent 6.0%, PVA: SA 1:1 (3.0%), CaCl2 4.0%, and bacterium: embedding agent (PVA-SA) 3:4; And the G5 microbeads produced at the optimal condition exhibited better cultivable bacteria count, encapsulation rate, expansion rate and mechanical strength. Pot experiment showed that G5 microbeads significantly increased the length and diameter of root and stem, and dry weight of P. nil during experimental stage under drought and salt stress. G5 microbeads also increased the total cultivable bacteria population, the activities of invertase (INV), urease (URE), phosphatase (PHO) and catalase (CAT), and the contents of available nitrogen (AN) and available phosphorus (AP) in the rhizosphere soil of P. nil. Therefore, our study obtained the optimal process of G5 microbeads, and confirmed its effect on improved plant growth and soil chemical and biological properties of P. nil. Thus it can be used as sustainable tool for eco-friendly bio-inoculants at salinity soil within arid and semi-arid areas. [Display omitted] •This study uses PVA-SA as a carrier to encapsulate PGPB for the first time, and optimum process was optimized by orthogonal experiment.•In the pot experiment, Bacillus pumilus G5 microbeads could affect soil environment to alleviate adverse effects by drought and salt stress on the growth of P. nil seedlings.