In vitro display evolution of unnatural peptides spontaneously cyclized via intramolecular nucleophilic aromatic substitutions

We report novel, ribosomally incorporatable, and intramolecularly cysteine-reactive fluorobenzoic acid-derived linkers for SELEX of mRNA-displayed unnatural peptides, which spontaneously cyclized intramolecular nucleophilic aromatic substitutions forming thioethers. With this strategy we identified...

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Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2022-04, Vol.58 (34), p.5237-5240
Hauptverfasser:	Yokoyama, Takumi, Ando, Takehiro, Takamori, Yukio, Fuji, Daisuke, Sato, Masashi, Vedi, Santhana, Yamamoto, Mizuki, Kawakami, Takashi
Format:	Artikel
Sprache:	eng
Schlagworte:	Cysteine - chemistry Peptides Peptides - chemistry Peptides, Cyclic - chemistry Proprotein Convertase 9 RNA, Messenger
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	We report novel, ribosomally incorporatable, and intramolecularly cysteine-reactive fluorobenzoic acid-derived linkers for SELEX of mRNA-displayed unnatural peptides, which spontaneously cyclized intramolecular nucleophilic aromatic substitutions forming thioethers. With this strategy we identified several novel PCSK9-binding peptides.
ISSN:	1359-7345 1364-548X
DOI:	10.1039/d2cc00584k