Protective effects of the biofloc system in Pacific white shrimp (Penaeus vannamei) culture against pathogenic Vibrio parahaemolyticus infection

The mitigation of vibriosis in shrimp culture could be accomplished by reducing the virulence of the pathogen or by increasing the immune response of the shrimp. This study aims to evaluate the application of a biofloc system in protecting Pacific white shrimp (Penaeus vannamei) from pathogenic Vibrio parahaemolyticus infection. Shrimp post-larvae (PL 20) with an average body weight of 0.041 ± 0.019 g were reared in an aquarium with a working volume of 33 L at a density of 3 L−1 for 21 days using two rearing systems, i.e., the biofloc system and the regular clear water system as control. In each system, the shrimp post-larvae were challenged by adding V. parahaemolyticus at different densities, 103, 105, and 107 CFU mL−1, while the negative control was performed by maintaining shrimp post-larvae in the clear water system without the addition of V. parahaemolyticus. The results of the in vitro experiment showed that the density and biofilm activity of V. parahaemolyticus reared in biofloc suspension was lower than that of the positive control (p