Stable reference gene selection for quantitative real-time PCR normalization in passion fruit (Passiflora edulis Sims.)
Background Passiflora edulis is a tropical fruit with high nutrient and medicinal values that is widely planted in southern China. However, the molecular biology of P. edulis has not been well studied. There are few reports regarding the choice of reference genes for gene expression studies of passi...
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description | Background
Passiflora edulis
is a tropical fruit with high nutrient and medicinal values that is widely planted in southern China. However, the molecular biology of
P. edulis
has not been well studied. There are few reports regarding the choice of reference genes for gene expression studies of passion fruit.
Methods and results
By using three algorithms, implemented in geNorm, NormFinder and BestKeeper, we have selected ten candidate reference genes to explore their transcriptional expression stability in various tissues and under cold stress conditions.
EF1
and
HIS
were stably expressed in five tissues.
Ts
and
OTU
were stably in vegetative organs.
50 S
and
Liom
were stably in reproductive organs. The transcriptional abundance of
EF1
and
UBQ
was stable in cold-treated and recovery treated leaf samples of
P. edulis
. In all samples,
EF1
and
Ts
exhibited the highest expression stability. Evaluation of selected genes using simple statistical methods (ANOVA and post hoc analysis). Overall,
EF1
emerged as the optimum reference gene for qRT-PCR normalize in
P. edulis
. In addition, the qRT-PCR analysis revealed that expression of
ICE1
increases with the duration of cold treatment.
Conclusions
In this study, we successfully screened stable reference genes from 10 candidates in
P. edulis
and verified the results by analyzing the expression level of
ICE1
. The results provide reliable and effective reference genes for future research on gene expression analysis in
P. edulis
, and lay a foundation for follow-up research on functional genes in
P. edulis
. |
doi_str_mv | 10.1007/s11033-022-07382-5 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2645857214</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2686429264</sourcerecordid><originalsourceid>FETCH-LOGICAL-c305t-5ac83bd4869e0770a40e2acb00a47ea3b7ad0d76e5b3a43b15f0687a0bac590e3</originalsourceid><addsrcrecordid>eNp9kc1u1DAURi1ERYfCC7BAlti0C5frvzizRKNSkCq1orC2bpKbylXiTO0EVJ4ez0wBiQUr2_L5vmv5MPZGwrkEcO-zlKC1AKUEOF0rYZ-xlbROC7N29XO2Ag1SmNrKY_Yy53sAMNLZF-xYW21dpcyK_bidsRmIJ-opUWyJ31Eknmmgdg5T5P2U-MOCcQ4zzuH7jsRBzGEkfrP5wuOURhzCT9zDIfIt5rzPpSXM_PRmd-yHKSGnbhlC5rdhzOdnr9hRj0Om10_rCfv28eLr5pO4ur78vPlwJVoNdhYW21o3namrNYFzgAZIYdtA2TlC3TjsoHMV2Uaj0Y20PVS1Q2iwtWsgfcJOD73bND0slGc_htzSMGCkacleVcbW1ilpCvruH_R-WlIsrytUXRm1LnCh1IFq05Rz-Ta_TWHE9Ogl-J0Wf9Diixa_1-JtCb19ql6akbo_kd8eCqAPQC5X8Y7S39n_qf0FifeY5w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2686429264</pqid></control><display><type>article</type><title>Stable reference gene selection for quantitative real-time PCR normalization in passion fruit (Passiflora edulis Sims.)</title><source>SpringerNature Journals</source><creator>Zhao, Meiqi ; Fan, Hang ; Tu, Zhonghua ; Cai, Guojun ; Zhang, Limin ; Li, Anding ; Xu, Meng</creator><creatorcontrib>Zhao, Meiqi ; Fan, Hang ; Tu, Zhonghua ; Cai, Guojun ; Zhang, Limin ; Li, Anding ; Xu, Meng</creatorcontrib><description>Background
Passiflora edulis
is a tropical fruit with high nutrient and medicinal values that is widely planted in southern China. However, the molecular biology of
P. edulis
has not been well studied. There are few reports regarding the choice of reference genes for gene expression studies of passion fruit.
Methods and results
By using three algorithms, implemented in geNorm, NormFinder and BestKeeper, we have selected ten candidate reference genes to explore their transcriptional expression stability in various tissues and under cold stress conditions.
EF1
and
HIS
were stably expressed in five tissues.
Ts
and
OTU
were stably in vegetative organs.
50 S
and
Liom
were stably in reproductive organs. The transcriptional abundance of
EF1
and
UBQ
was stable in cold-treated and recovery treated leaf samples of
P. edulis
. In all samples,
EF1
and
Ts
exhibited the highest expression stability. Evaluation of selected genes using simple statistical methods (ANOVA and post hoc analysis). Overall,
EF1
emerged as the optimum reference gene for qRT-PCR normalize in
P. edulis
. In addition, the qRT-PCR analysis revealed that expression of
ICE1
increases with the duration of cold treatment.
Conclusions
In this study, we successfully screened stable reference genes from 10 candidates in
P. edulis
and verified the results by analyzing the expression level of
ICE1
. The results provide reliable and effective reference genes for future research on gene expression analysis in
P. edulis
, and lay a foundation for follow-up research on functional genes in
P. edulis
.</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-022-07382-5</identifier><identifier>PMID: 35357624</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animal Anatomy ; Animal Biochemistry ; Biomedical and Life Sciences ; Fruits ; Gene expression ; Histology ; Life Sciences ; Medicinal plants ; Morphology ; Original Article ; Passiflora edulis ; Reproductive organs</subject><ispartof>Molecular biology reports, 2022-07, Vol.49 (7), p.5985-5995</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2022</rights><rights>2022. The Author(s), under exclusive licence to Springer Nature B.V.</rights><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2022.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c305t-5ac83bd4869e0770a40e2acb00a47ea3b7ad0d76e5b3a43b15f0687a0bac590e3</citedby><cites>FETCH-LOGICAL-c305t-5ac83bd4869e0770a40e2acb00a47ea3b7ad0d76e5b3a43b15f0687a0bac590e3</cites><orcidid>0000-0002-6573-9098</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11033-022-07382-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11033-022-07382-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35357624$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Meiqi</creatorcontrib><creatorcontrib>Fan, Hang</creatorcontrib><creatorcontrib>Tu, Zhonghua</creatorcontrib><creatorcontrib>Cai, Guojun</creatorcontrib><creatorcontrib>Zhang, Limin</creatorcontrib><creatorcontrib>Li, Anding</creatorcontrib><creatorcontrib>Xu, Meng</creatorcontrib><title>Stable reference gene selection for quantitative real-time PCR normalization in passion fruit (Passiflora edulis Sims.)</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>Mol Biol Rep</addtitle><description>Background
Passiflora edulis
is a tropical fruit with high nutrient and medicinal values that is widely planted in southern China. However, the molecular biology of
P. edulis
has not been well studied. There are few reports regarding the choice of reference genes for gene expression studies of passion fruit.
Methods and results
By using three algorithms, implemented in geNorm, NormFinder and BestKeeper, we have selected ten candidate reference genes to explore their transcriptional expression stability in various tissues and under cold stress conditions.
EF1
and
HIS
were stably expressed in five tissues.
Ts
and
OTU
were stably in vegetative organs.
50 S
and
Liom
were stably in reproductive organs. The transcriptional abundance of
EF1
and
UBQ
was stable in cold-treated and recovery treated leaf samples of
P. edulis
. In all samples,
EF1
and
Ts
exhibited the highest expression stability. Evaluation of selected genes using simple statistical methods (ANOVA and post hoc analysis). Overall,
EF1
emerged as the optimum reference gene for qRT-PCR normalize in
P. edulis
. In addition, the qRT-PCR analysis revealed that expression of
ICE1
increases with the duration of cold treatment.
Conclusions
In this study, we successfully screened stable reference genes from 10 candidates in
P. edulis
and verified the results by analyzing the expression level of
ICE1
. The results provide reliable and effective reference genes for future research on gene expression analysis in
P. edulis
, and lay a foundation for follow-up research on functional genes in
P. edulis
.</description><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Fruits</subject><subject>Gene expression</subject><subject>Histology</subject><subject>Life Sciences</subject><subject>Medicinal plants</subject><subject>Morphology</subject><subject>Original Article</subject><subject>Passiflora edulis</subject><subject>Reproductive organs</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kc1u1DAURi1ERYfCC7BAlti0C5frvzizRKNSkCq1orC2bpKbylXiTO0EVJ4ez0wBiQUr2_L5vmv5MPZGwrkEcO-zlKC1AKUEOF0rYZ-xlbROC7N29XO2Ag1SmNrKY_Yy53sAMNLZF-xYW21dpcyK_bidsRmIJ-opUWyJ31Eknmmgdg5T5P2U-MOCcQ4zzuH7jsRBzGEkfrP5wuOURhzCT9zDIfIt5rzPpSXM_PRmd-yHKSGnbhlC5rdhzOdnr9hRj0Om10_rCfv28eLr5pO4ur78vPlwJVoNdhYW21o3namrNYFzgAZIYdtA2TlC3TjsoHMV2Uaj0Y20PVS1Q2iwtWsgfcJOD73bND0slGc_htzSMGCkacleVcbW1ilpCvruH_R-WlIsrytUXRm1LnCh1IFq05Rz-Ta_TWHE9Ogl-J0Wf9Diixa_1-JtCb19ql6akbo_kd8eCqAPQC5X8Y7S39n_qf0FifeY5w</recordid><startdate>20220701</startdate><enddate>20220701</enddate><creator>Zhao, Meiqi</creator><creator>Fan, Hang</creator><creator>Tu, Zhonghua</creator><creator>Cai, Guojun</creator><creator>Zhang, Limin</creator><creator>Li, Anding</creator><creator>Xu, Meng</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6573-9098</orcidid></search><sort><creationdate>20220701</creationdate><title>Stable reference gene selection for quantitative real-time PCR normalization in passion fruit (Passiflora edulis Sims.)</title><author>Zhao, Meiqi ; Fan, Hang ; Tu, Zhonghua ; Cai, Guojun ; Zhang, Limin ; Li, Anding ; Xu, Meng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c305t-5ac83bd4869e0770a40e2acb00a47ea3b7ad0d76e5b3a43b15f0687a0bac590e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Fruits</topic><topic>Gene expression</topic><topic>Histology</topic><topic>Life Sciences</topic><topic>Medicinal plants</topic><topic>Morphology</topic><topic>Original Article</topic><topic>Passiflora edulis</topic><topic>Reproductive organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Meiqi</creatorcontrib><creatorcontrib>Fan, Hang</creatorcontrib><creatorcontrib>Tu, Zhonghua</creatorcontrib><creatorcontrib>Cai, Guojun</creatorcontrib><creatorcontrib>Zhang, Limin</creatorcontrib><creatorcontrib>Li, Anding</creatorcontrib><creatorcontrib>Xu, Meng</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Meiqi</au><au>Fan, Hang</au><au>Tu, Zhonghua</au><au>Cai, Guojun</au><au>Zhang, Limin</au><au>Li, Anding</au><au>Xu, Meng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stable reference gene selection for quantitative real-time PCR normalization in passion fruit (Passiflora edulis Sims.)</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><addtitle>Mol Biol Rep</addtitle><date>2022-07-01</date><risdate>2022</risdate><volume>49</volume><issue>7</issue><spage>5985</spage><epage>5995</epage><pages>5985-5995</pages><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>Background
Passiflora edulis
is a tropical fruit with high nutrient and medicinal values that is widely planted in southern China. However, the molecular biology of
P. edulis
has not been well studied. There are few reports regarding the choice of reference genes for gene expression studies of passion fruit.
Methods and results
By using three algorithms, implemented in geNorm, NormFinder and BestKeeper, we have selected ten candidate reference genes to explore their transcriptional expression stability in various tissues and under cold stress conditions.
EF1
and
HIS
were stably expressed in five tissues.
Ts
and
OTU
were stably in vegetative organs.
50 S
and
Liom
were stably in reproductive organs. The transcriptional abundance of
EF1
and
UBQ
was stable in cold-treated and recovery treated leaf samples of
P. edulis
. In all samples,
EF1
and
Ts
exhibited the highest expression stability. Evaluation of selected genes using simple statistical methods (ANOVA and post hoc analysis). Overall,
EF1
emerged as the optimum reference gene for qRT-PCR normalize in
P. edulis
. In addition, the qRT-PCR analysis revealed that expression of
ICE1
increases with the duration of cold treatment.
Conclusions
In this study, we successfully screened stable reference genes from 10 candidates in
P. edulis
and verified the results by analyzing the expression level of
ICE1
. The results provide reliable and effective reference genes for future research on gene expression analysis in
P. edulis
, and lay a foundation for follow-up research on functional genes in
P. edulis
.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>35357624</pmid><doi>10.1007/s11033-022-07382-5</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-6573-9098</orcidid></addata></record> |
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subjects | Animal Anatomy Animal Biochemistry Biomedical and Life Sciences Fruits Gene expression Histology Life Sciences Medicinal plants Morphology Original Article Passiflora edulis Reproductive organs |
title | Stable reference gene selection for quantitative real-time PCR normalization in passion fruit (Passiflora edulis Sims.) |
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