Stable reference gene selection for quantitative real-time PCR normalization in passion fruit (Passiflora edulis Sims.)
Background Passiflora edulis is a tropical fruit with high nutrient and medicinal values that is widely planted in southern China. However, the molecular biology of P. edulis has not been well studied. There are few reports regarding the choice of reference genes for gene expression studies of passi...
Gespeichert in:
Veröffentlicht in: | Molecular biology reports 2022-07, Vol.49 (7), p.5985-5995 |
---|---|
Hauptverfasser: | , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | Background
Passiflora edulis
is a tropical fruit with high nutrient and medicinal values that is widely planted in southern China. However, the molecular biology of
P. edulis
has not been well studied. There are few reports regarding the choice of reference genes for gene expression studies of passion fruit.
Methods and results
By using three algorithms, implemented in geNorm, NormFinder and BestKeeper, we have selected ten candidate reference genes to explore their transcriptional expression stability in various tissues and under cold stress conditions.
EF1
and
HIS
were stably expressed in five tissues.
Ts
and
OTU
were stably in vegetative organs.
50 S
and
Liom
were stably in reproductive organs. The transcriptional abundance of
EF1
and
UBQ
was stable in cold-treated and recovery treated leaf samples of
P. edulis
. In all samples,
EF1
and
Ts
exhibited the highest expression stability. Evaluation of selected genes using simple statistical methods (ANOVA and post hoc analysis). Overall,
EF1
emerged as the optimum reference gene for qRT-PCR normalize in
P. edulis
. In addition, the qRT-PCR analysis revealed that expression of
ICE1
increases with the duration of cold treatment.
Conclusions
In this study, we successfully screened stable reference genes from 10 candidates in
P. edulis
and verified the results by analyzing the expression level of
ICE1
. The results provide reliable and effective reference genes for future research on gene expression analysis in
P. edulis
, and lay a foundation for follow-up research on functional genes in
P. edulis
. |
---|---|
ISSN: | 0301-4851 1573-4978 |
DOI: | 10.1007/s11033-022-07382-5 |