A lysine sensor for process control

A lysine sensor for process control of lysine fermentation was developed based on a Clark‐type electrode in combination with L ‐lysine‐α‐oxidase. The enzyme, isolated from Trichoderma viride , was immobilized between a cellulose and a polypropylene foil using a polyurethane resin. Lysine determinati...

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Veröffentlicht in:	Journal of chemical technology and biotechnology (1986) 1994-03, Vol.59 (3), p.279-287
Hauptverfasser:	Siegler, Klaus, Weber, Bettina, Weber, Ekkehard, Tonder, Karin, Klingner, Edith, Riechert, Frank
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences biosensor Biosensors Biotechnology Fundamental and applied biological sciences. Psychology L-lysinoxidase lysine fermentation lysine oxidase electrode Methods. Procedures. Technologies process control Various methods and equipments
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Beschreibung
Zusammenfassung:	A lysine sensor for process control of lysine fermentation was developed based on a Clark‐type electrode in combination with L ‐lysine‐α‐oxidase. The enzyme, isolated from Trichoderma viride , was immobilized between a cellulose and a polypropylene foil using a polyurethane resin. Lysine determinations were carried out in a flow‐through system as anodic measurements when H 2 O 2 was measured and as chathodic measurements when the consumption of O 2 was followed. The sensitivity of the sensor toward other amino acids was determined.
ISSN:	0268-2575 1097-4660
DOI:	10.1002/jctb.280590311