Bioaccessibility of phospholipids in homogenized goat milk: Lipid digestion ecology through INFOGEST model

•The disruption and recombination of MFGM promoted digestibility of homogenized PC.•Casein attached to the surface of MFGM improving the stability of homogenized milk.•Present accurately quantified profile of PCs in vitro digestion via phospholipidome.•Homogenized milk with MFG showed 280 nm particle size and interacted with casein.•Goat milk subjected to homogenization at 30 MPa showed optimal PC bioavailability. Phospholipids-rich goat milk provides health benefits to consumers. The effects of homogenization on the disruption and recombination of milk fat globule membrane and change the fatty acid positional distribution in glycerophospholipids profile by phosphatidylcholine metabolism pathways were investigated. Goat milk was homogenized at different intensity pressure. Homogenized samples were introduced into harmonized INFOGEST digestion model. Results showed that phosphatidylcholine increased significantly during storage in 30 MPa and were approximately twice that in raw milk (LOD 0.27–1.49 μg/L and LOQ 0.89–4.92 μg/L, respectively). Meanwhile, both linoleic acid (C18:2) and α-linolenic acid (C18:3ω-3), the foremost polyunsaturated acyl chains in homogenized milk extracts, showed upward trends. Notably, homogenization increased the number and altered the composition of Sn-1, 2 diacylglycerols via increasing trypsin and pancreatic lipase (PLRP2, MAUC15, CD36 and BSSL) expression and accelerated the phosphatidylcholine conversion. Ultimately, the relationship between homogenization and milk fat globule recombination and phospholipids bioaccessibility was preliminary established.