Chemical structure and antioxidant activity of a neutral polysaccharide from Asteris Radix et Rhizoma
Asteris Radix et Rhizoma (AR) has been widely used as a herbal medicine for treating various symptoms and possesses a number of bioactivities. A neutral polysaccharide ARP-1 was isolated from AR with weight-average molecular weight of 214 kDa. The heteropolysaccharide ARP-1 was composed of fucose, a...
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Veröffentlicht in: | Carbohydrate polymers 2022-06, Vol.286, p.119309-119309, Article 119309 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Asteris Radix et Rhizoma (AR) has been widely used as a herbal medicine for treating various symptoms and possesses a number of bioactivities. A neutral polysaccharide ARP-1 was isolated from AR with weight-average molecular weight of 214 kDa. The heteropolysaccharide ARP-1 was composed of fucose, arabinose, galactose, glucose and mannose with a molar ratio of 0.40:14.25:10.22:1.06:0.41. Linkage and NMR analysis showed that ARP-1 had a backbone containing →3,6)-β-d-Galp-(1→ and →6)-β-d-Galp-(1 → residues, and oligosaccharide side chains containing Araf and Galp units were attached to the backbone at C-3 of →3,6)-β-d-Galp-(1 → residues. Antioxidant activity assays showed that ARP-1 exhibited potent antioxidant activities, including ABTS, hydroxy and DPPH radicals scavenging and reducing power. Besides, ARP-1 decreased the production of ROS and MDA, and improved the activities of SOD, which resulted in the protection of PC12 cells against H2O2-induced oxidative stress. The findings indicated that ARP-1 might be used as a potential natural antioxidant.
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ISSN: | 0144-8617 1879-1344 |
DOI: | 10.1016/j.carbpol.2022.119309 |