Fractionation of fungal pectin enzymes by immobilised metal ion affinity chromatography

Fractionation of fungal pectin enzymes by immobilised metal ion affinity chromatography

IMAC on Sepharose-iminodiacetic acid-Cu(II) was used to separate pectin lyase and pectinesterase from a commercial pectic enzyme. Pectin lyase did not bind to the chromatographic matrix at pH 8.0, while pectinesterase was retained and only eluted when the pH of the buffer was brought down to 3.0. Po...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of the science of food and agriculture 1994-04, Vol.64 (4), p.527-531
Hauptverfasser: Navarro del Canizo, A, Hours, R A, Miranda, M V, Cascone, O
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:IMAC on Sepharose-iminodiacetic acid-Cu(II) was used to separate pectin lyase and pectinesterase from a commercial pectic enzyme. Pectin lyase did not bind to the chromatographic matrix at pH 8.0, while pectinesterase was retained and only eluted when the pH of the buffer was brought down to 3.0. Polygalacturonase activity was found in both fractions thus suggesting that IMAC can discriminate between 2 forms of that enzyme. (Original abstract-amended)
ISSN:0022-5142