Determination of xanthine using a ratiometric fluorescence probe based on boron-doped carbon quantum dots and gold nanoclusters
A dual-emission ratiometric fluorescent sensing system based on boron-doped carbon quantum dots (B-CQDs) and gold nanoclusters (AuNCs) has been developed for the determination of xanthine. The blue fluorescence of B-CQDs at 445 nm is then reduced by the AuNCs through the inner filter effect (IFE) un...
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Veröffentlicht in: | Mikrochimica acta (1966) 2022-04, Vol.189 (4), p.148-148, Article 148 |
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Sprache: | eng |
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Zusammenfassung: | A dual-emission ratiometric fluorescent sensing system based on boron-doped carbon quantum dots (B-CQDs) and gold nanoclusters (AuNCs) has been developed for the determination of xanthine. The blue fluorescence of B-CQDs at 445 nm is then reduced by the AuNCs through the inner filter effect (IFE) under a single excitation wavelength of 370 nm. By the catalysis of xanthine oxidase (XOD), xanthine is oxidized by oxygen dissolved in the solution to produce H
2
O
2
. The horseradish peroxidase (HRP) catalyzes H
2
O
2
to generate hydroxyl radicals, which can quench the fluorescence of AuNCs, leading to the recovery of the fluorescence of B-CQDs. Based on the relationship between the fluorescence intensity ratio (
F
445
/
F
665
) and the concentration of xanthine, the designed method exhibits a good linearity range of 1.2–500.0 μmol L
−1
and a limit of detection of 0.37 μmol L
−1
. The ratiometric fluorescent is applied to determine xanthine in human urine samples. Good recoveries of spiked samples in the range 99.2–105.0% are obtained by the proposed assay, with relative standard deviations (RSD) ranging from 0.9 to 2.6%.
Graphical abstract |
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ISSN: | 0026-3672 1436-5073 |
DOI: | 10.1007/s00604-021-05139-8 |