Identification of potential genes in endobronchial tuberculosis after bronchoscopic cryotherapy by transcriptome sequencing

Bronchoscopic cryotherapy facilitates the treatment of endobronchial tuberculosis (EBTB) and helps suppress progressive bronchial stenosis. However, the molecular mechanism of bronchoscopic cryotherapy in EBTB patients has not been reported. Transcriptome sequencing was performed to explore differentially expressed mRNAs (DEGs) in EBTB patients before and after bronchoscopic cryotherapy. Gene Ontology (GO) and KEGG analyses were carried out. Five genes (MKLN1, HIGD1A, PTGES, SKIL, and MCEMP1) were selected and validated using real-time qPCR (RT- qPCR). In transcriptome analysis, 448 DEGs with p 1 were identified; of these, 171 and 277 DEGs were significantly up- and down-regulated after bronchoscopic cryotherapy, respectively. Results displayed 337 biological process (GO-BP), 48 cellular component (GO-CC) and 62 molecular function (GO-MF) terms and 20 KEGG pathways. RT- qPCR results were consistent with the sequencing data. These data provide informative evidence and recommendations for further scientific research on bronchoscopic cryotherapy for EBTB. •We firstly identified transcriptome map of EBTB patients before and after bronchoscopy cryotherapy treatment.•The results showed that 448 DEGs (171 upregulated and 277 downregulated) were obtained between BCT group and ACT group.•These results provide a theoretical basis for further exploring the molecular mechanism of bronchoscopy cryotherapy for EBTB.