Dynamics and correlations in multiplex immune profiling reveal persistent immune inflammation in male drug users after withdrawal

[Display omitted] •Drug withdrawal elicits immune responses contributing to the development of withdrawal symptoms and relapse.•Broad and strong inflammatory responses occurred at the early stage after drug withdrawal.•Immune dysregulation still exists and may last longer in heroin and METH users after withdrawal for 12 months.•IL-7 may play an important role in regulating the immune responses after drug withdrawal. Drug withdrawal elicits immune responses that contribute to the development of withdrawal symptoms and relapse. The understanding of the immunologic dynamics after drug withdrawal is limited, precluding the finding of promising immune intervention measures. Here, we performed cytokine and multiplex immune profiling in heroin, methamphetamine (METH) and ephedrine users after withdrawal and identified the correlation between cytokines and other immune parameters. We showed that broad and strong inflammatory responses occurred at the early stage after drug withdrawal, and the inflammatory responses showed a downtrend with the extension of withdrawal time. Notably, immune dysregulation remained through and may last longer than 12 months after withdrawal in heroin and METH users. Our findings suggest that cytokines, immune cells, complement and immunoglobulin form a complex immune network that regulates immune responses after withdrawal. These data provide a reference for future scientific research and drug research and development.