Energy metabolism as the target of 3-phenyllactic acid against Rhizopus oryzae

3-phenyllactic acid (PLA) has broad anti-fungal activity, however, target sites of PLA on fungal cells and its anti-fungal mechanism of action have been poorly studied. In this study, we explored the inhibition mechanism of Rhizopus oryzae (R. oryzae) on rotten lily bulbs by PLA. The minimum inhibit...

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Veröffentlicht in:	International journal of food microbiology 2022-05, Vol.369, p.109606-109606, Article 109606
Hauptverfasser:	Fan, Wenguang, Li, Baoyu, Du, Nana, Hui, Tian, Cao, Yingying, Li, Xin, Ren, Haiwei
Format:	Artikel
Sprache:	eng
Schlagworte:	3-phenyllactic acid Acids Aconitic acid Adenosine diphosphate Adenylate kinase Anti-fungal mechanism Apoptosis ATP Cell membranes Citric acid Cytochrome Cytochrome-c oxidase Cytochromes Dehydrogenase Dehydrogenases Electron microscopy Energy Metabolism Enzymatic activity Enzyme activity Fungi Fungicides Gene expression Glycolysis Hexokinase Isocitric acid Ketoglutaric acid Kinases Lactates - metabolism Malic acid Metabolism Metabolites Metabolomics Microscopy Minimum inhibitory concentration Mitochondria Nicotinamide adenine dinucleotide Organelles Oxidative phosphorylation Oxoglutarate dehydrogenase (lipoamide) Phosphorylation Rhizopus - metabolism Rhizopus oryzae Scanning electron microscopy Transmission electron microscopy Tricarboxylic acid cycle Ultrastructure
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Zusammenfassung:	3-phenyllactic acid (PLA) has broad anti-fungal activity, however, target sites of PLA on fungal cells and its anti-fungal mechanism of action have been poorly studied. In this study, we explored the inhibition mechanism of Rhizopus oryzae (R. oryzae) on rotten lily bulbs by PLA. The minimum inhibitory concentration value of PLA against R. oryzae was 8 mg/mL. We observed the ultrastructure of R. oryzae by scanning electron microscopy and transmission electron microscopy which indicated that PLA did not damage the cell membrane, but destroyed the mitochondria and other organelles. Tandem mass tag proteomes showed that PLA significantly down-regulated (P
ISSN:	0168-1605 1879-3460
DOI:	10.1016/j.ijfoodmicro.2022.109606