Physiological, morphological and transcriptomic responses of Tibetan naked carps (Gymnocypris przewalskii) to salinity variations

Gymnocypris przewalskii is a native cyprinid fish that dwells in the Lake Qinghai with salinity of 12–13‰. It migrates annually to the freshwater rivers for spawning, experiencing the significant changes in salinity. In the present study, we performed the physiological, morphological and transcriptomic analyses to understand the osmoregulation in G. przewalskii. The physiological assay showed that the osmotic pressure of G. przewalskii was almost isosmotic to the brackish lake water. The low salinity reduced its ionic concentrations and osmotic pressure. The plasticity of gill microstructure was linked to the salinity variations, including the presence of mucus and intact tight junctions in brackish water and the development of the mitochondria-rich cells and the loosened tight junctions in freshwater. RNA-seq analysis identified 1926 differentially expressed genes, including 710 and 1216 down- and up-regulated genes in freshwater, which were enriched in ion transport, cell-cell adhesion, and mucus secretion. Genes in ion uptake were activated in low salinity, and mucus pathways and tight junction showed the higher transcription in brackish water. The isosmoticity between the body fluid and the environment suggested G. przewalskii was in the metabolic-saving condition in the brackish water. The decreased salinity disrupted this balance, which activated the ion uptake in freshwater to maintain osmotic homeostasis. The gill remodeling was involved in this process through the development of the mitochondria-rich cells to enhance ion uptake. The current finding provided insights into the potential mechanisms of G. przewalskii to cope with salinity alteration. [Display omitted] •Decreased salinity caused the reduction of ionic and osmotic concentration in Gymnocypris przewalskii•Low salinity led to gill remodeling in G. przewalskii, including the appearance of mitochondrial-rich cell.•The intact tight junction and the accumulation of mucus granules were observed in gill of G. przewalskii under high salinity.•The transcriptomic analysis demonstrated the genes of ion uptake functions were activated in G. przewalskii subjected to low salinity.•DEGs were overrepresented in pathways of cell-cell junction, response to osmotic stress and mucus secretion.