Biodegradation of nitriles derived from glucosinolates in rapeseed meal by BnNIT2: a nitrilase from Brassica napus with wide substrate specificity

Nitriles derived from glucosinolates (GSLs) in rapeseed meal (RSM) can cause lesions on animal liver and kidneys. Nitrilase converts nitriles to carboxylic acids and NH 3 , eliminating their toxicity. Here we describe a nitrilase, Bn NIT2, from Brassica napus (optimal temperature, 45 °C; pH, 7.0) th...

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Veröffentlicht in:	Applied microbiology and biotechnology 2022-04, Vol.106 (7), p.2445-2454
Hauptverfasser:	Zhang, Heng, Zhang, Honghai, Qin, Xing, Wang, Xiaolu, Wang, Yuan, Tu, Tao, Wang, Yaru, Yao, Bin, Huang, Huoqing, Luo, Huiying
Format:	Artikel
Sprache:	eng
Schlagworte:	Aminohydrolases Ammonia Analysis Animals Biodegradation Biomedical and Life Sciences Biotechnologically Relevant Enzymes and Proteins Biotechnology Brassica Brassica napus Brassica napus - metabolism Brassica rapa - metabolism Carboxylic acids Detoxification Enzymes Glucosinolates Glucosinolates - metabolism Identification and classification Iron Kidneys Life Sciences Methods Microbial Genetics and Genomics Microbiology Mutagenesis Mutants Nitrilase Nitriles Nitriles - metabolism pH effects Plasmids Properties Rape (Plant) Rape plants Rapeseed Substrate Specificity Substrates Thermal stability Toxicity
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