Echinicola arenosa sp. nov., isolated from marine sand

A novel bacterium, designated CAU 1574 T , was isolated from marine sand. Cells were Gram stain negative, aerobic, gliding and rod shaped. Growth was observed at 20–37 °C (optimum, 30 °C), a pH of 5.5–10.0 (optimum, 8.0), and 0–3.0% (w/v) NaCl concentrations (optimum, 1%). Based on the results of 16S rRNA gene sequence analyses, strain CAU 1574 T belonged to the genus Echinicola , and showed the highest similarity to Echinicola shivajiensis JCM 17847 T (97.5%). Phylogenomic analysis based on consisting of 92 core genes extracted from the genome sequences showed that strain CAU 1574 T was affiliated with species in the genus Echinicola . The average nucleotide identity (ANI), average amino acid identity (AAI), and digital DNA–DNA hybridization (dDDH) values between strain CAU 1574 T and the closely related species were below the cut-off values of 95–96, 90, and 70%, respectively used for species demarcation. The chemotaxonomic data of CAU 1574 T were as follows: major isoprenoid quinone, MK-7; predominant polar lipids, phosphatidylethanolamine, two unidentified aminophospholipids and two unidentified lipids; major fatty acids, iso-C 15:0 , summed feature 3 (C 16:1 ω 6 c /C 16:1 ω 7 c ). The 5.4 Mb genome included 20 contigs and 4237 protein-coding genes with a 39.8 mol% G + C content. Based on the phylogenetic, phenotypic, and physiological result, strain CAU 1574 T represents a novel species of this genus Echinicola , for which the name Echinicola arenosa sp. nov. is proposed. The type strain is CAU 1574 T (= KCTC 82410 T = MCCC 1K05669 T ).