Nitrate fate and decadal shift impacted by land use change in a rural karst basin as revealed by dual nitrate isotopes

Nitrate pollution in oxygenated karst aquifers is common due to nitrification and anthropogenic inputs. However, the shift of nitrogen sources influenced by enhanced rural tourism activities and land use changes are not well understood. In this study, hydrochemistry and dual nitrate isotopes of water samples from a rural karst basin in Chongqing, southwestern China were employed to investigate the nitrate fate and its decadal change during the periods from 2007–2008 and 2017–2019. The results showed that δ15N–NO3 and δ18O–NO3 values at the groundwater basin resurgence averaged 9 ± 3.4‰ and 2.5 ± 3.4‰, respectively, with a mean NO3− concentration of 19.7 ± 5.4 mg/L in 2017–2019, clearly exceeding natural background levels. The dual isotope results suggested that nitrification occurred at the sampled sites. From 2007–2008 to 2017–2019, the mean δ15N–NO3 values from the primary sink point and the resurgence of the underground river water samples increased from −0.2 ± 2.1 to 11.2 ± 4.8‰, 4.2 ± 0.9 to 9.0 ± 3.4‰, respectively. A Bayesian mixing model in R (MixSIAR) based on the isotopes revealed that soil organic nitrogen, and manure and sewage proportions for the groundwater increased by 34% and 23%, respectively, while chemical fertilizer and atmospheric precipitation proportions decreased by 32% and 25%, respectively. These decadal changes resulted from reforestation practices and enhanced rural tourism activities in the basin, which were evidenced by the change of land use patterns. The elevated nitrogen load from the rapid development of rural tourism is likely to increase this contamination in the near future if the infrastructure cannot meet the demands. The results from this study could contribute to minimizing environmental health risks in drinking water when rural tourism activities are increasing. [Display omitted] •Nitrate fate and decadal shift in a typical karst aquifer system were studied.•Nitrification was dominant due to oxidic conditions.•Proportions of soil organic nitrogen and manure and sewage increased the most.•Elevated manure and sewage source was a result of rural tourism activities.•Rural tourism is likely to increase nitrate contamination in the near future.