Unveiling the characterization and development of prokaryotic community during the start-up and long-term operation of a pilot-scale anaerobic membrane bioreactor for the treatment of real municipal wastewater

The anaerobic membrane bioreactor (AnMBR) is a promising sustainable process and technology for the treatment of municipal wastewater from the perspective of carbon neutrality. In this study, a large pilot-scale AnMBR was constructed and the microbial community development of the anaerobic digested sludge in the AnMBR was determined during the treatment of municipal wastewater. The AnMBR system was conducted for 217 days during a long-term operation with the feed of real municipal wastewater. The characterization and dynamics of the microorganisms revealed that a stable prokaryotic community was gradually achieved. In the community of methane-producing archaea (or methanogens), the acetotrophic methanogen Methanosaeta was significantly enriched at an ambient temperature of 25 °C with an overwhelming relative abundance in the entire community. The abundance of Methanosaeta was even higher than the most abundant bacterial phyla Chloroflexi, Firmicutes, Proteobacteria and Bacteroidetes. This phenomenon is quite different from that found in other typical anaerobic systems. The massive enrichment of methanogens is the key to maintaining stable methane production in the treatment of municipal wastewater by the AnMBR. The interspecies cooperation of major functional bacterial groups including protein/carbohydrate/cellulose-degrading (genera Anaerovorax, Aminomonas, Levilinea, Flexilinea and Ruminococcus etc.), sulfate-reducing (Desulfovibrio and Desulfomicrobium etc.) and syntrophic (Syntrophorhabdus and Syntrophus etc.) bacteria with acetotrophic and hydrogenotrophic archaea enhances the stability of reactor operation and help to acclimate the entire prokaryotic community to the characteristics of real municipal wastewater. [Display omitted] •A pilot-scale AnMBR succeeded in the treatment of municipal wastewater at 25 °C.•Microbial community structure was investigated during start-up and operation.•A stable prokaryotic community was cultivated with real municipal wastewater.•The population of acetotrophic methanogen was even higher than bacterial genera.•Cooperation of major functional bacterial groups enhanced the stability of AnMBR.