Inhibiting tricin biosynthesis improves maize lignocellulose saccharification

Lignin is a technological bottleneck to convert polysaccharides into fermentable sugars, and different strategies of genetic-based metabolic engineering have been applied to improve biomass saccharification. Using maize seedlings grown hydroponically for 24 h, we conducted a quick non-transgenic approach with five enzyme inhibitors of the lignin and tricin pathways. Two compounds [3,4-(methylenedioxy)cinnamic acid: MDCA and 2,4-pyridinedicarboxylic acid: PDCA] revealed interesting findings on root growth, lignin composition, and saccharification. By inhibiting hydroxycinnamoyl-CoA ligase, a key enzyme of phenylpropanoid pathway, MDCA decreased the lignin content and improved saccharification, but it decreased root growth. By inhibiting flavone synthase, a key enzyme of tricin biosynthesis, PDCA decreased total lignin content and improved saccharification without affecting root growth. PDCA was three-fold more effective than MDCA, suggesting that controlling lignin biosynthesis with enzymatic inhibitors may be an attractive strategy to improve biomass saccharification. •We screened enzyme inhibitors for their ability to improve maize saccharification.•3,4-(Methylenedioxy)cinnamic acid (MDCA) inhibited lignin formation.•2,4-Pyridinedicarboxylic acid (PDCA) inhibited tricin formation and thus lignin.•Both compounds increased saccharification, but PDCA was more effective.•Controlling enzyme inhibition may improve biomass saccharification.