Hand-powered vacuum-driven microfluidic gradient generator for high-throughput antimicrobial susceptibility testing
The growth of bacterial resistance to antimicrobials is a serious problem attracting much attention nowadays. To prevent the misuse and abuse of antimicrobials, it is important to carry out antibiotic susceptibility testing (AST) before clinical use. However, conventional AST methods are relatively...
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Veröffentlicht in: | Biosensors & bioelectronics 2022-06, Vol.205, p.114100-114100, Article 114100 |
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Sprache: | eng |
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Zusammenfassung: | The growth of bacterial resistance to antimicrobials is a serious problem attracting much attention nowadays. To prevent the misuse and abuse of antimicrobials, it is important to carry out antibiotic susceptibility testing (AST) before clinical use. However, conventional AST methods are relatively laborious and time-consuming (18–24 h). Here, we present a hand-powered vacuum-driven microfluidic (HVM) device, in which a syringe is used as the only vacuum source for rapid generating concentration gradient of antibiotics in different chambers. The HVM device can be preassembled with various amounts of antibiotics, lyophilized, and stored for ready-to-use. Bacterial samples can be loaded into the HVM device through a simple suction step. With the assistance of Alamar Blue, the AST assay and the minimum inhibitory concentration (MIC) of different antibiotics can be investigated by comparing the growth results of bacteria in different culture chambers. In addition, a parallel HVM device was proposed, in which eight AST assays can be performed simultaneously. The results of MIC of three commonly used antibiotics against E. coli K-12 in our HVM device were consistent with those obtained by traditional method while the detection time was shortened to less than 8 h. We believe that our platform is high-throughput, cost-efficient, easy to use, and suitable for POCT applications.
•The HVM device can generate stable and customizable concentration gradients by hand-powered vacuum strategy within 40 s.•The parallel device can simultaneously perform eight AST assays and directly obtain the MIC of antibiotics within 8 h.•The device is simple in equipment and operation, and short in culture time, suitable for rapid AST and POCT applications. |
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ISSN: | 0956-5663 1873-4235 |
DOI: | 10.1016/j.bios.2022.114100 |