Streptomyces corallincola and Kineosporia corallincola sp. nov., two new coral-derived marine actinobacteria

Two new marine actinobacteria, designated as J2-1 and J2-2 , were isolated from a coral, , collected from Rayong Province, Thailand. The taxonomic positions of the two strains were identified based on polyphasic taxonomy. Based on morphological characteristics and chemotaxonomy, strains J2-1 and J2-2 were identified as members of the genus and , respectively. Strains J2-1 and J2-2 showed the highest 16S rRNA gene sequence similarity to T44 (98.62 %) and VN05A0415 (98.08 %), respectively. Strain J2-1 had chemotaxonomic properties resembling members of the genus . ll-Diaminopimelic acid, glucose and ribose were detected in the whole-cell hydrolysate. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositolmannoside, unidentified aminolipid and five unidentified phospholipids were detected as the polar lipids. The major cellular fatty acids were C iso, C anteiso, C iso, C , C anteiso, C iso and C iso. Strain J2-2 a showed similar cell composition to members of the genus . Both isomers of ll- and -diaminopimelic acid were detected in the peptidoglycan. Arabinose, galactose, madurose and xylose were observed in the whole-cell hydrolysate. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, phosphatidylcholine, an unidentified phospholipid and an unidentified glycolipid. The major cellular fatty acids were C , C ω9 , C 10-methyl, tuberculostearic acid, C and C . Both strains could be distinguished from their closely related type strains according to their phenotypic characteristics. Comparative genome analysis indicated the delineation of two novel species based on digital DNA-DNA hybridization and average nucleotide identity values, which were below 70 and 95 %, respectively. The names proposed are sp. nov. (J2-1 =TBRC 13503 =NBRC 115066 ) and sp. nov. (J2-2 =TBRC 13504 =NBRC 114885 ).