Increasing the bioactivity of kynurenine by ultraviolet irradiation via resonance energy transfer in vitro

Kynurenine (Kyn) is involved in a variety of physiological/pathological reactions via activating aryl hydrocarbon receptor (Ahr). However, how to activate Ahr by Kyn under physiological/pathological conditions is still unclear. Here, we presented that Kyn (8 μM, a concentration less than the dose of Kyn-induced Ahr activation) significantly induced the nuclear transfer of Ahr and the expression of cytochrome P450 1A1 (CYP1A1, a classic biomarker for Ahr activation) when co-administered with ultraviolet (UV) irradiation in 95D cells, which were transfected transiently with siRNA against indoleamine 2,3-dioxygenase 1 (IDO 1) and cultured in cell medium supplemented with bovine serum containing bovine serum albumin (BSA), in vitro. Additionally, we found that the fluorescence intensity of BSA was attenuated by Kyn (2, 4, 6, 8, 10, 12 and 14 μM) mainly through quenching the fluorescence of tryptophan (Trp) residues in the pattern of dynamic quenching related to molecular diffusion. More important, resonance energy transfer from excited-state BSA to Kyn was confirmed, leading to the generation “energetic” Kyn that might be ability of hyperactivity according to the theory of photochemical reaction. These data indicate that UV irradiation is contributable for Kyn to function, and present a novel pattern of altering the activity of biomolecules to some degree. [Display omitted] •Low dose of Kyn significantly induced the expression of cytochrome P450 1A1 when co-administered with ultraviolet irradiation.•Kyn attenuated the fluorescence intensity of BSA mainly through quenching the fluorescence of Trp residues.•Kyn-induced reduction of fluorescence intensity of BSA belonged to dynamic quenching related to molecular diffusion.•It occurred for resonance energy transfer between Kyn and excited-state BSA leading to the generation of “energetic” Kyn.