Ultrasensitive assay for saliva-based SARS-CoV-2 antigen detection

Ultrasensitive assay for saliva-based SARS-CoV-2 antigen detection

Widespread SARS-CoV-2 testing is invaluable for identifying asymptomatic/pre-symptomatic individuals. There remains a technological gap for highly reliable, easy, and quick SARS-CoV-2 diagnostic tests suitable for frequent mass testing. Compared to nasopharyngeal (NP) swab-based tests, saliva-based...

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Veröffentlicht in:Clinical chemistry and laboratory medicine 2022-04, Vol.60 (5), p.771-777
Hauptverfasser: Ren, Annie, Sohaei, Dorsa, Ulndreaj, Antigona, Pons-Belda, Oscar D., Fernandez-Uriarte, Amaia, Zacharioudakis, Ioannis, Sigal, George B., Stengelin, Martin, Mathew, Anu, Campbell, Christopher, Padmanabhan, Nikhil, Romero, Daniel, Joe, Jessica, Soosaipillai, Antoninus, Kulasingam, Vathany, Mazzulli, Tony, Li, Xinliu A., McGeer, Allison, Diamandis, Eleftherios P., Prassas, Ioannis
Format: Artikel
Sprache:eng
Schlagworte:
Antigens
Antigens, Viral
Coronaviruses
COVID-19
COVID-19 - diagnosis
COVID-19 Testing
Electrochemiluminescence
electrochemiluminescence (ECL) immunoassay
Humans
Immunoassay
mass testing
nasopharyngeal (NP) swab
Nasopharynx
Nucleocapsids
Saliva
Saliva - chemistry
SARS-CoV-2
sensitivity
Sensitivity analysis
Sensitivity and Specificity
Severe acute respiratory syndrome
Severe acute respiratory syndrome coronavirus 2
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Zusammenfassung:Widespread SARS-CoV-2 testing is invaluable for identifying asymptomatic/pre-symptomatic individuals. There remains a technological gap for highly reliable, easy, and quick SARS-CoV-2 diagnostic tests suitable for frequent mass testing. Compared to nasopharyngeal (NP) swab-based tests, saliva-based methods are attractive due to easier and safer sampling. Current saliva-based SARS-CoV-2 rapid antigen tests (RATs) are hindered by limited analytical sensitivity. Here, we report one of the first ultrasensitive, saliva-based SARS-CoV-2 antigen assays with an analytical sensitivity of
ISSN:1434-6621
1437-4331
DOI:10.1515/cclm-2021-1142