Shedding Light on ecDNA Behavior Using CRISPR-Based Live-Cell Imaging

Shedding Light on ecDNA Behavior Using CRISPR-Based Live-Cell Imaging

Extrachromosomal oncogene amplification on extrachromosomal DNA (ecDNA) has emerged as a hallmark of many cancers. In this issue, Yi and colleagues developed a CRISPR-based method for imaging ecDNA in live cells, termed ecTag. Using ecTag, the authors reveal important features of ecDNA in cancer cel...

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Veröffentlicht in:Cancer discovery 2022-02, Vol.12 (2), p.293-295
1. Verfasser: Henssen, Anton G
Format: Artikel
Sprache:eng
Schlagworte:
Gene Amplification
Humans
Neoplasms - genetics
Oncogenes
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Zusammenfassung:Extrachromosomal oncogene amplification on extrachromosomal DNA (ecDNA) has emerged as a hallmark of many cancers. In this issue, Yi and colleagues developed a CRISPR-based method for imaging ecDNA in live cells, termed ecTag. Using ecTag, the authors reveal important features of ecDNA in cancer cells such as their random mitotic segregation and clustering into transcriptionally active hubs after mitosis. .
ISSN:2159-8274
2159-8290
DOI:10.1158/2159-8290.CD-21-1525