High‐Efficiency Capture of Cells by Softening Cell Membrane

The capture of circulating tumor cells (CTCs) by nanostructured substrate surface is a useful method for early diagnosis of cancer. At present, most methods used to improve the cell capture efficiency are based on changing substrate surface properties. However, there are still some gaps between these methods and practical applications. Here, a method is presented for improving cell capture efficiency from a different perspective, that is, changing the properties of the cells. Concretely, the mechanical properties of the cell membrane are changed by adding Cytochalasin D to soften the cell membrane. Furthermore, a corresponding theoretical model is proposed to explain the experimental results. It is found that cell softening can reduce the resistance of cell adhesion, which makes the adhesion ability stronger. The high‐efficiency capture of cells by softening the cell membrane provides a potential method to improve the detection performance of CTCs. Softening the cell membrane by adding Cytochalasin D can improve the capture efficiency of cells on the surface of nanostructured substrate. The reason for the improvement is the decrease of the stretching modulus of the cell membrane due to the destruction of filamentous actin by Cytochalasin D, which can reduce the resistance of cell adhesion.